View source for Part:BBa K4343082


__NOTOC__
<partinfo>BBa_K4343082 short</partinfo>

IgASE1 gene encodes ∆-9 elongase from Isochrysis galbana. This enzyme can catalyze the elongation of C18:2 to C20:2 in Y. lipolytica.

===Characterisation===
We integrated IgASE1 controlled by the TEF promoter to the SCP2 site of the genome of Y. lipolytica through this segment. Then we detected the proportion of C18:2 and C20:2 by gas chromatography (GC) and test the activity of ∆-9 elongase according to the results.

===Result===
The conversion of C18:2 to C20:2 under the catalytic action of IgASE1 was around 26.3%, with only 16.5% of C20:2 produced.

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4343082 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K4343082 parameters</partinfo>
<!-- -->