View source for Part:BBa K4167000


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<partinfo>BBa_K4167000 short</partinfo>

Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples. 
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===Usage and Biology===


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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4167000 SequenceAndFeatures</partinfo>
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To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows: 
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[[File:K4167000-fig.1.jpg|center]]
Fig.1 The map of toehold switch-amilCP described by SnapGene Viewer, showing the restriction enzyme information (no EcoRI and PstI sites). 
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After detecting the restriction enzyme information of toehold switch-amilCP using SnapGene software, it was inserted into the pSB1C3 plasmid to construct the standard part pSB1C3-toehold switch-amilCP with PCR method. Then it was identified as follows:
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[[File:K4167000-fig.2.jpg|center]]

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===Functional Parameters===
<partinfo>BBa_K4167000 parameters</partinfo>
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