View source for Part:BBa K3788017


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<partinfo>BBa_K3788017 short</partinfo>

<p>This part is constituted from  -35 and -10 boxes, the +& transcription site, a LexA box and the beggining of the CDS of <i>caa</i>.
The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the <i>caa-cal</i> operon.</p>
<p>This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.</p>



===Role in natural environment===

<p>Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon <i>caa-cal</i>. <i>caa</i> gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by <i>cai</i> gene (-). <i>cal</i> gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. </p>


===Experiment design===

To characterize the <i>caa-cal</i> promoter, we made a transcriptionnal fusion between <i>caa</i> and GFP coding sequence (Part:BBa_E0040) before of the timer-lysis device (Part:BBa_K3788018) that we put in the Registry as Part:BBa_K3788019.
This construction, according to what is known from this system functionment, is supposed to express GFP in presence of mitomycinA, that induces the SOS response by damaging DNA.

With the construction BBa_K3788019 we could determine whether the promoter is well responding to SOS response induced by DNA damages.



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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K3788017 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K3788017 parameters</partinfo>
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