View source for Part:BBa K3332087


__NOTOC__
<partinfo>BBa_K3332087 short</partinfo>

We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter

With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.
===Usage and Biology===
This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.
<html>
    <figure>
        <img src="https://2020.igem.org/wiki/images/0/0f/T--XMU-China--XMU-China_2020-pTrc-2_derivative_B0034_ecfp_B0015.png" width="40%" style="float:center">
        <figcaption>
        <p style="font-size:1rem">
        </p>
        </figcaption>
    </figure>
</html>
'''Fig 1. '''pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015)

===Characterization===
The agarose gel electrophoresis images are shown as below: 
[[File:T--XMU-CHINA--BBa K3332087.png|none|500px|caption]]
'''Fig 2.''' pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by ''Xba'' I and ''Pst'' I (about 1018 bp)

===Reference===
[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. &apos;Deadman&apos; and &apos;Passcode&apos; microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K3332087 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K3332087 parameters</partinfo>
<!-- -->