View source for Part:BBa K3113102


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<partinfo>BBa_K3113102 short</partinfo>

This sequence codes for the loop structure coiled-coil 9 which interacts with coiled-coil 10 and allows us to specifically load proteins and RNA into vesicles.

<h2>Usage</h2>

To increase the modularity of our system we decided to test coiled-coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles.

<h2>Biology</h2>

We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo.<ref>Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094.</ref>


<h2>Characterization</h2>



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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K3113102 SequenceAndFeatures</partinfo>

<h2>References</h2>

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===Functional Parameters===
<partinfo>BBa_K3113102 parameters</partinfo>
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