View source for Part:BBa K2996709


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<partinfo>BBa_K2996709 short</partinfo>

LuxI is put downstream of BBa_K2996705, as a reporter, which is a synthetase for acyl-homoserine lactones (AHL).
dcas9 binding to lure means the occurrence of off-target incidence. RpoA, which is linked to dcas9, will recruits RNA polymerase and the gene downstream of the promotor will be activated. 
We develop a way to record off-target incidence, with LuxI gene under the control of promotor J23117. Increased expression of luxI enzyme will synthesis enough AHL molecules, which can penetrate to the bacteria capable of information storage. 





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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2996709 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2996709 parameters</partinfo>
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