View source for Ribosome Binding Sites/Prokaryotic/Constitutive/Anderson

==Description==
Parts J61100-J61139 are a family of ribosome binding site parts identified from a saturation mutagenic library.  They are suitable for general protein expression applications in ''E. coli''.

{|class="wikitable" style="border:3px solid black; width: 60%" align="center"  cellspacing="0"
! style="background:grey; color:black"  |Identifier
! style="background:grey; color:black"  |Sequence<sup>a</sup>
! style="background:grey; color:black"  |Strength<sup>b</sup>
|-
| align="center"| Master Sequence
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">NNN</font>GA<font color="red">NNN</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61100</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61101</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">CAG</font>GA<font color="red">CCC</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61102</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">TCC</font>GA<font color="red">TGT</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61103</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">TTA</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61104</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">AGG</font>GA<font color="red">CAG</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61105</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61106</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61107</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61108</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61109</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61110</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61111</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61112</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61113</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61114</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61115</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61116</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61117</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61118</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61119</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61120</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61121</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61122</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61123</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61124</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61125</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61126</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61127</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61128</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61129</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61130</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61131</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61132</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61133</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61134</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61135</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61136</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61137</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61138</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|
|-
|align="center"|<partinfo>J61139</partinfo>
|<font face="Courier" size="+1"><font color="grey">TCTAGAG</font>AAAGA<font color="red">GGG</font>GA<font color="red">CAA</font><font color="grey">TACTAG</font><font color="green">ATG</font></font>
|}

These parts are present in plasmid pSB1A2, but there is also a constitutive promoter (J23100-derived) inserted into the XbaI site.  So, for example, the EcoRI/PstI region of part J61100 reads:
  Biobrick 5'    XbaI                    J23100              XbaI    '''RBS Part'''     Biobrick 3'
  gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''GAAAGAGGGGACAA'''actagtagcggccgctgcag

This feature in no way prevents the use of these parts in standard Biobrick assembly.  Normal prefix insertion into EcoRI/XbaI will delete this promoter element.  Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion.

Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely 
"T".  The "G" downstream of the XbaI site obeys the standard.  Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.

More on this family of parts and their quantitative behavior is described [[JCA_Arkin_RBSFamilyPart2 | here]].