View source for Part:BBa K3198008


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<partinfo>BBa_K3198008 short</partinfo>

This part contains the arabinose inducible promoter pBAD and antitoxin component HicB of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death.

===Description===
This part contains the antitoxin component of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death.

===Usage===
Team NUS Singapore 2019 has added a new biobrick (BBa_K3198008) into the iGEM repository this year. This biobrick was found to possess the ability to neutralize the effect of BBa_K3198000 and therefore functions as an antitoxin. For this reason, team NUS Singapore 2019 used this biobrick as part of their sleep-wake module to control the growth of E. coli. More specifically, to overcome the pre-induced dormant state of these cells.

===Biology===
HicB is from hicAB locus of Escherichia coli K-12. HicB functions as an mRNA interferase antitoxin. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. HicB neutralizes HicA and therefore functions as an antitoxin. HicB could resuscitate cells inhibited by HicA.



===References===
Jorgensen, M. G., Pandey, D. P., Jaskolska, M., & Gerdes, K. (2008). HicA of Escherichia coli Defines a Novel Family of Translation-Independent mRNA Interferases in Bacteria and Archaea. Journal of Bacteriology, 191(4), 1191–1199. doi: 10.1128/jb.01013-08
<br><br>Maisonneuve, E., Shakespeare, L. J., Jørgensen, M. G., & Gerdes, K. (2011). Bacterial persistence by RNA endonucleases. Proceedings of the National Academy of Sciences, 108(32), 13206–13211. doi: 10.1073/pnas.1100186108


===Source===
BBa_K3198001 originated from E. coli K12 and its sequence was synthesized by IDT.

===Design Considerations===


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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K3198008 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K3198008 parameters</partinfo>
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