View source for Part:BBa K2656004

__NOTOC__
<partinfo>BBa_K2656004 short</partinfo>
[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]

Part BBa_K2656004 is the constitutive low promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106] adapted into the Golden Gate grammar. Thus, it is compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid] assembly methods. 

Following the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>, it can be combined with other GB compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI Type IIS one-pot reaction.

Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656105 BBa_K2656105], which was used in a comparative promoters expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656104 BBa_K2656104] and [https://parts.igem.org/Part:BBa_K2656107 BBa_K2656107]. 
They all were assembled in a Golden Braid alpha1 plasmid using the same RBS, CDS and terminator:

<html>
<ul>
	<li></html>RBS [https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:B0030 B0030] RBS in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
	<li></html>CDS [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:E0040 E0040] GFPmut3b sequence in its Golden Braid standardized version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
	<li></html>Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
</ul>
</html>










By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so rationale choose its optimized values based for each promoter tested.

[[File:T--Valencia_UPV_promoter_UPV2018.png|900px|thumb|none|alt=RBS experiment.|Figure 1. Promoter expression experiment with  BBa_K2656004, K2656005 and BBa_K2656007 basic pieces]]


{|class='wikitable'
|colspan=4|Table 1. Optimized parameters for the BBa_K2656004 promoter. 
|-
|'''Parameter'''
|'''Value'''
|-
|Constitutive transcription rate CR
|CR = 171.15 min-1
|-
|Dilution rate μ
|μ = 0.01001 min-1
|}

We have also calculated the relative force between the different promoters, taking [https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005 promoter] as a reference. Likewise, a ratio of the promoter strength (p parameter) for the different promoters refer to the stronger one (reference) has been calculated. 

{|class='wikitable'
|colspan=4|Table 2. BBa_K2656004 relative strength and p ratio.
|-
|'''Parameter'''
|'''Value'''
|-
|Relative strength
|0.2603
|-
|p parameter ratio 
|0.3172
|}


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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2656004 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2656004 parameters</partinfo>
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