View source for Part:BBa K2633006


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<partinfo>BBa_K2633006 short</partinfo>

This organism is a transformable gram-negative bacterium which was isolated from surface-sterilized switchgrass (''Panicum virgatum'') roots. It is capable of reinfecting roots of ''Brachypodium distachyon''. ''Enterobacter ludwigii'' can be transformed using [http://2018.igem.org/Team:MichiganState/Experiments electroporation]. It is susceptible to chloramphenicol and streptinomycin. Contact Julian Liber (liberjul@msu.edu) if a sample of this strain is desired.

MichiganState 2018 transformed this chassis using plasmids with the[https://parts.igem.org/Part:pSB1C3 pSB1C3 backbone], this first documented use of this plasmid in an ''Enterobacter'' species. The part we designed for expression of an ''acdS''-eGFP fusion is [https://parts.igem.org/Part:BBa_K2633000 BBa_K2633000], which allows for fluorescent microscopy of the bacteria ''in situ'' and expression of the plant growth promoting enzyme ACC deaminase.

The 16S rRNA gene sequence can be found [https://static.igem.org/mediawiki/parts/5/51/T--MichiganState--FCP2-01_16S_rRNA_gene.txt here]. The Hsp60 sequence can be found [https://static.igem.org/mediawiki/2018/3/35/T--MichiganState--FCP2-01_Hsp60.txt here].

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2633006 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2633006 parameters</partinfo>
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