View source for Part:BBa K2558207


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<partinfo>BBa_K2558207 short</partinfo>

This construct is the CRISPRi device, which is composed of dCas9, Ptac controlled gRNA targeting PHIF repressible promotor (BBa_K1725000) and constitutively (BBa_J23100, Anderson medium promotor) expressed lacI protein. We designed this device along with K2558206 and K2558208, which are the same except for the strength of the constitutive lacI expression. We aim to test the repression efficiency of our CRISPRi system with the help of PHIF repressible promotor. 

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2558207 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2558207 parameters</partinfo>
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