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<b>LasR-pLas-GFP</b><br>
Group: <b>Shanghaitech iGEM 2017</b>

The HSL receiver LasR from P.aeruginosa activates expression of GFP protein in response to 3OC12HSL. 

This Receiver can be easily replaced by other AHL receivers in our collection. A full collection could be found in: (link tobe added!!!!!!!!)

==<b>Usage and Biology</b>==

[[File:LasRpLasGFPPARTS.png|thumb|center|700px|<b>Fig. 1 Genetic Circuit Design</b>]]

When AHL is added with concentration higher than a critical value, the constitutively expressed LasR will bind to the AHL molecule 3OC12HSL, dimerize and bind to the pLas regulatory sequence to activate GFP expression.


==<b>Fluorescent Response to cognate 3OC12-HSL</b>==
To test this part, we used standard 3OC12HSL (HSL produced by LasI in P.aeruginosa) to determine the response curve. 

(Figure2&3-response to 3OC12HSL to be added)

==Orthogonality test against non-cognate inducers==
We have characterized crosstalk response of LasR to several non-cognate AHLs:
[[File:https://static.igem.org/mediawiki/parts/7/78/T--Shanghaitech--LasRorthogonality.png|thumb|center|1400px|<b>Fig. Orthogonality test of LasR-pLas</b>]]
(Figure4-7-response to non-cognate inducers to be added)

==<b>Usages in our Project</b>==
We developed a measurement protocol using the fluorescent protein coupled AHL receiver germs to measure the actual AHLs concentration in high precision and sensitivity in compared with LC-MS.


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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2315011 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2315011 parameters</partinfo>
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