View source for Part:BBa K2371017


__NOTOC__
<partinfo>BBa_K2371017 short</partinfo>

The echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) is a fusion gene resulting from chromosome inversion and was identified in nonsmall cell lung cancer(NSCLC).The EML4-ALK fused gene has two variants (A and B).Using computer stimulation, we selected seven sgRNA binding sites from all the combination of possible sites. Four sgRNA binding sites on Variant A and three sgRNA binding sites on Variant B.

[[File:BGIC-Union Part sgRNA Figure6.png|700px]]
<h5>Figure 1. The four sgRNA binding sites of fusion gene EML4-ALK Variant A. We designed four sgRNA binding sites in target gene – sgRNA23, sgRNA33, sgRNA83 and sgRNA93. The number of them indicates the location of their first base pair in target gene. The combination of sgRNA binding sites will be based on the rule that a pair of sgRNA should be distributed in EML4 or ALK area separately or one of them located on the connecting area of the EML4-ALK fusion gene.</h5>

[[File:BGIC-Union Part sgRNA Figure7.png|700px]]
<h5>Figure 2. The three sgRNA binding sites of fusion gene EML4-ALK Variant B. We designed three sgRNA binding sites in target gene – sgRNA70, sgRNA126 and sgRNA147. The number of them indicates the location of their first base pair in target gene. The combination of sgRNA binding sites will be based on the rule that a pair of sgRNA should be distributed in EML4 or ALK area separately or one of them located on the connecting area of the EML4-ALK fusion gene.</h5>

This part is coding sequence of sgRNA 23 mentioned above.

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2371017 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K2371017 parameters</partinfo>
<!-- -->