View source for Part:BBa K2368009


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<h1>Introduction</h1>
<partinfo>BBa_K2368009 short</partinfo>
<p> This part sweetness receptor T1R2 fusion with the HIS tag, just like the picture showed below. </p>
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[[File:T-BIT-China-2017parts-38.png|center|500px|默认文字]]
<p style="text-align: center">Fig.1 The schematic diagram of HIS+T1R2 overlap</p>


<h1>Design</h1>
<p> In order to show whether the sweet receptor T1R2 are translated and located correctly, we fusion the HIS tag to N-terminal of T1R2. The reason fused tag to N-terminal is that the C-terminal of T1R2 interacts with the G protein α subunit, which is a crucial factor during signal deliver through G protein signal pathway. And the N-terminal is outside the cell, so it can be detected without killing it, using immunofluorescence can figure out whether T1R2 is located at the right position. </p>


[[File:T-BIT-China-2017parts-39.png|center|500px|默认文字]]
<p style="text-align: center">Fig.2 Electrophoresis of HIS+T1R2 overlap. </p>

<h1>Experiment</h1>
<p> At the beginning, we construct the specific primer that consists of the gene sequence of HIS tag. Then, fused T1R2 with the HIS using PCR. The length of sequence is 50bp. </p>
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===Usage and Biology===

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<h2>Sequence and Features</h2>
<partinfo>BBa_K2368009 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2368009 parameters</partinfo>
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