View source for Cell-free chassis/Commercial E. coli T7 S30

=Commercial ''E. coli'' T7 S30=

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||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free Systems</font>]
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 <font face=georgia color=#000066 size=4>Chassis description</font>]
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30/Optimum_Temp <font face=georgia color=#000066 size=4>Optimum temperature</font>]
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30/Peak_Time <font face=georgia color=#000066 size=4>Peak time</font>]
||<font face=georgia color=#000066 size=4>Product stability</font>
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30/Expression_Capacity <font face=georgia color=#000066 size=4>Expression capacity</font>]
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30/Expression_Lifespan <font face=georgia color=#000066 size=4>Expression lifespan</font>]
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==Description==
The Commercial ''E. coli'' T7 S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). '''''E. coli'' strain B''' deficient in OmpT endoproteinase and lon protease activity was used.

The simple constitutive gene expression device [https://parts.igem.org/wiki/index.php/Part:BBa_E7104 BBa_E7104] has been used to characterize this cell-free chassis

==References==
Please refer to the [http://www.promega.com/tbs/tb219/tb219.pdf Promega cell extract user guide] for detailed information about the Commercial ''E. coli'' T7 S30 extract.