View source for Part:BBa K2120418


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<partinfo>BBa_K2120418 short</partinfo>


This is one of our application circuit.

This circuit consists of two parts:the inhibitor device and reporter device ,which have already been made into basic parts of biobricks.
We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI ,
a reporter protein-mRFP (BBa_E1010) was used as a signal to represent if the concentration of inhibitor controlled by different promoters can repress the expression of downstream gene controllled by inducible promoter completely .At first,we test fluorescence intensity of mRFP protein expressed by each circuit in high copy plasmid pSB1K3 ,the results show that inhibitor devices with J23116 and J23109 cannot inhibit the expression of mRFP ,which means that the strength of these two promoters is too weak to turn off the reporter device in our circuit.


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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K2120418 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K2120418 parameters</partinfo>
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