View source for Part:BBa K1688004

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<partinfo>BBa_K1688004 short</partinfo>

Composite part consisting of the RBS, BBa_B0034, and the red fluorescent protein, dTomato (BBa_K1688019). dTomato has not been bricked previously.

<partinfo>BBa_K1688004 SequenceAndFeatures</partinfo>
[[File:uppsala_dtomato.jpg]]

Figure 1. dTomato expressed in ''E. coli'' DH5-alpha with an 
inducible promoter.


== Usage and Biology==

The dTomato protein is a fluorescent protein dimer, created by direct evolution of the wild-type DsRed, from Discosoma sp. (Shaner et al, - Improved monomeric red, orange and yellow fluorescent proteins derived from ''Discosoma sp.'' red fluorescent protein, 2004). The dTomato protein is a fluorescent dimer protein that emits orange-red light when it is excited by green-yellow light. It is preferable to use – especially in self-made fluorometry tests – because the excitation wavelengths and the emission wavelengths don't overlap as much as in other fluorescent proteins. The dTomato excitation peak is at 554 nm and 50% of it is at 510 nm. Also, its emission peak is at 581 nm and its 50% emission at 629 nm (Figure 2).

                          [[File:Uppsala2015_dTomato_Chromatogram.png]]
Figure 2. Excitation (blue curve) and emission (red curve) spectra for the dTomato fluorescent protein (the graph is designed with the following tool: https://www.chroma.com/spectra-viewer)

== References ==
Nathan C Shaner, Robert E Campbell, Paul A Steinbach, Ben N G Giepmans, Amy E Palmer & Roger Y Tsien. “Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein”, 2004