View source for Part:BBa K1684003

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<partinfo>BBa_K1684003 short</partinfo>

Yellow chromoprotein optimized for E.coli K12 with the standar RBS

===Usage and Biology===

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version with the standar RBS is codon optimized for the expression in E.coli K12.

===Results===

The Figure 1  present different samples of parts used for transformation of competent cells composed by a RBS (BBa_B0034) and the optimized chromoprotein sequence amilGFP (BBa_K1684001 ). 

The agarose gel shown in Line 1, the negative control; Line 2, belongs to the purified DNA of pGLO (5371 bp), was run as a positive control. Line 5 and Line 6 contain duplicate BBa_K1684003 (BBa_B0034 + BBa_K1684001) biobrick; however, a positive result is observed only in Line 5, with size of 2822 bp, while in line 6 only presents contamination, without the presence of any genetic material.


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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1684003 SequenceAndFeatures</partinfo>

===References===

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680.
doi:10.1371/journal.pone.0002680


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===Functional Parameters===
<partinfo>BBa_K1684003 parameters</partinfo>
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