View source for Part:BBa J100198

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<partinfo>BBa_J100198 short</partinfo>

Cloned in the fitness gene tester construct ([[Part:BBa_J100199]]), part J100198 contains the fitness gene thymidylate synthase [[Part:BBa_J100132]]. J100198 contains theophylline riboswitch D downstream of T5, a constitutive promoter [[Part:BBa_J100065]]. The construct is built to be used in thymine auxotrophs (''thyA-'') that necessitate added thymine in the growth media for cell division. Upon successful binding of theophylline on the riboswitch (we used 0.3mM), thymidylate synthase mRNA is translated for prototophic growth and cellular division in the ''thyA-'' chassis. J100198 was cloned into pSB1A8 [[Part:BBa_J119043]]. <br><br>

See Experience page [[Part:BBa_J100198:Experience]] for details about how well this device works. 


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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_J100198 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_J100198 parameters</partinfo>
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