View source for Part:BBa K1321327

__NOTOC__
<partinfo>BBa_K1321327 short</partinfo>

This is a purple chromoprotein gfasPurple (BBa_K1033917) in pSEVA331-Bb plasmid backbone (part BBa_K1321300). This construct is a member of the ''G.xylinus'' genetic engineering toolkit (parts BBa_K1321295 - BBa_K1321332).




''G.xylinus'' toolkit was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium ''Gluconacetobacter xylinus'' (parts Bba_K1321305 and BBa_K1321306). As no registry parts had been tested in ''G.xylinus'', the aim of this toolkit was to determine the parts usable in ''G.xylinus'' and to characterize them in this host. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in ''G.xylinus'' and ''E.coli'' (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for ''G.xylinus'' engineering.

NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in ''Gluconacetobacter'' species, the ''G.xylinus'' genetic engineering toolkit is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the Registry. However, in order to make the ''G.xylinus'' toolkit available for the synthetic biology community, Imperial iGEM 2014 team has made it freely available upon request, with quality control provided (see Experience). To request, please contact Imperial iGEM 2014 team.

<!-- Add more about the biology of this part here
===Usage and Biology===

<!-- -->
<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1321327 SequenceAndFeatures</partinfo>


<!-- Uncomment this to enable Functional Parameter display 
===Functional Parameters===
<partinfo>BBa_K1321327 parameters</partinfo>
<!-- -->