View source for Part:BBa K1321126

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<partinfo>BBa_K1321126 short</partinfo>

Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.


This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-SmtA-part-table.PNG]]

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1321126 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K1321126 parameters</partinfo>
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