View source for Part:BBa K1172912

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<partinfo>BBa_K1172912 short</partinfo>

Part 1 of the Biosystem M (pRha TetR alr)

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===Usage and Biology===


<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1172912 SequenceAndFeatures</partinfo>
==Results==
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==='''Characterization of the tetracycline operator TetO'''===

[[File:Team-Bielefeld-Biosafeyt-System_TetORalive-TetOGFP-OD.jpg|600px|thumb|center|'''Figure 12:''' Characterization of the bacterial growth of the TetO promoter with GFP (<bbpart>BBa_E0040</bbpart>) in the Biosafety-Strain K-12 ∆alr ∆dadX. The M9 media was supplemented with 5 mM D-alanine. It can be seen, that the bacteria grow faster on M9 minimal media glucose than on M9 minimal media glycerol. ]]

[[File:Team-Bielefeld-Biosafety-System-Tet2Flourescence.jpg|600px|thumb|center|'''Figure 13:''' Characterization of the fluorescence of TetO promoter with GFP (<bbpart>BBa_E0040</bbpart>) in the Biosafety-Strain K-12 ∆alr ∆dadX. The bacteria were cultivated on M9 minimal media with 5 mM D-alanine supplemented..]]


[[File:Team-Bielefeld-Biosafeyt-System-TetORalive-specTetGFP.jpg|600px|thumb|center|'''Figure 14:''' Specific growth rate of GFP behind the TetO promoter by the use of different carbon sources.]]

==='''Characterization of the Biosafety-System TetOR alive'''===

[[File:Team-Bielefeld-Biosafety-System-TetORalive-ODALL.jpg|600px|thumb|center|'''Figure 15:''' Characterization of the bacterial growth of the Biosafety-System on M9 minimal media glycerol. The figure compares the wild tpye K-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.]]


[[File:Team-Bielefeld-Biosafety-System-TetORalive-FluorALL.jpg|600px|thumb|center|'''Figure 16:''' Characterization of the fluorescence of the Biosafety-System TetOR alive. The figure compares the wild tpye k-12 and the Biosafety-Strain K-12 ∆alr ∆dadX and the induction by L-Rhamnose to pure glycerol.]]

[[File:Team-Bielefeld-Biosafety-System-TetORalive-sepzProductALL.jpg|600px|thumb|center|'''Figure 17:''' Specific production rate, calculated by equation (1).]]


==='''Conclusion of the Results'''===

[[File:Team-Bielefeld-Biosafety-System-TetORalive-Overview.jpg|600px|thumb|center|'''Figure 18:''' Comparision of the specific production rate of GFP in the with L-Rhamnose induced Biosafety-System TetOR alive, the uninduced Biosafety-System TetOR alive and the second part of the Biosafety-System (TetO-GFP only).]]


==References==

Saenger W. et al. (2000):Der Tetracyclin-Repressor – das Musterbeispiel für einen biologischen Schalter, In:[http://onlinelibrary.wiley.com/doi/10.1002/1521-3757%2820000616%29112:12%3C2122::AID-ANGE2122%3E3.0.CO;2-8/abstract Angewandte Chemie Volume 112, Issue 12, pages 2122–2133]
===Functional Parameters===
<partinfo>BBa_K1172912 parameters</partinfo>
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