View source for Part:BBa M0052

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<partinfo>BBa_M0052 short</partinfo>

This sequence codes for the amino acid sequence AANDENYADAS, a variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to moderately fast degradation through SspB-mediated binding to the ClpX protease. DAS variants of the SsrA tag are dependent on SspB for efficient degradation.

Note that tagged protein degradation rates are dependent on concentration of proteases (ClpX, ClpA) and binding mediators (SspB). Degradation rates are also heavily dependent on protein stability and depend on Km of binding to the protease, which is highly variable with each substrate. Be aware of ClpX, ClpA, and SspB knockouts when using  SsrA tags; some expression strains have these mutations.

The following rates of degradation of respective tagged proteins were observed when this tag was linked to the C-terminal of the protein: ~1 Vmax/ [Clpx6] min-1 tagged to Arc, from (1).

See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240. 
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See improvement of this part at https://parts.igem.org/Part:BBa_K1051208.
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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_M0052 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_M0052 parameters</partinfo>
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