View source for Part:BBa K1031421

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<partinfo>BBa_K1031421 short</partinfo>
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<p>For detailed information concerning HcaR and Ph promoter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR">2013 Peking iGEM Biosensor HcaR</a></p>

<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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== '''Structure''' ==

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<img src="https://static.igem.org/mediawiki/igem.org/4/41/Peking2013_HcaRFig1.jpg" style="width:600px; margin-left:170px" />
<p style="text-align:center"><b>Fig 1</b> Structure of <i>Ph</i> promoter regulated by HcaR and chemical pathway. 
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== '''Sequence and Features''' ==


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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K1031421 SequenceAndFeatures</partinfo>



== '''Construction and data''' ==

For the biosensor circuit ''Ph''/HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. 
K1031421 is composed of three elements, ''Ph'' promoter activated by HcaR, RBS <html><a href="https://parts.igem.org/Part:BBa_B0032">B0032</a></html> and reporter gene sfGFP ('''Fig 1a'''). Induction ratio was tested when K1031421 is in collocation with J23106-HcaR and J23114-HcaR, as shown in the dashed boxes ('''Fig 1b'''). From data we concluded biosensor circuit ''Ph''/HcaR adopting ''Pc'' J23114 and RBS B0032 obtained the optimal performance. 

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<img src="https://static.igem.org/mediawiki/igem.org/thumb/c/ca/Peking2013_part_K1031421.png/800px-Peking2013_part_K1031421.png" style="width:600px; margin-left:170px" />
<p style="text-align:center"><b>Fig 1</b> Construction and tunning of reporter circuit <i>Ph</i>-B0032-sfGFP. (<b>a</b>) The orange arrow represents <i>Ph</i> promoter activated by HcaR. The green oval stands for RBS B0032. sfGFP coding sequence is shown with dark blue, while terminator B0015 is in dark red. (<b>b<b>) Test was done following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Horizontal axis represents biosensor circuit adopting different combination of <i>Pc</i> promoter and RBS. Vertical axis stands for induction ratio. The dashed boxes refer to data for reporter circuit <i>Pc</i>-B0032-sfGFP in collocation with biosensor circuits J23106-HcaR and J23114-HcaR respectively.
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===Functional Parameters===
<partinfo>BBa_K1031420