View source for Part:BBa K892800

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<partinfo>BBa_K892800 short</partinfo>

'''pLac I - LovTAP - Term - pTrp - TetR - Term - pTetO - sfGFP - Term'''

Part BBa_892800 is an improvement on part
BBa_K191003.  It fixes a frameshift deletion in LovTAP and includes
the inverter and sfGFP readout on a single plasmid. The hybrid protein
LovTAP has a light-sensitive domain (Lov: Light, oxygen, voltage) and
a regulatory domain (part of the E. coli Trp repressor). The fused
protein allows transmission of the conformational change induced by
light (on the light-sensitive domain) to the DNA-binding domain,
therefore resulting in an increase or decrease of the regulatory
domain's affinity for its DNA binding site. The activation of the
LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm.

For more details, please consult the section of LovTAP system on team
[http://2009.igem.org/Team:EPF-Lausanne/LOVTAP/ EPF-Lausanne wiki], or the [http://2012.igem.org/Team:Washington/Optogenetics Optogenetics page] on the
2012 University of Washington wiki.

'''Usage and Biology'''

In order to be able to test the efficiency of the fused protein
LovTAP, its expression is induced by IPTG (Due to the LacI-sensitive
promoter). The readout of the system is also included on the plasmid.
The TetR driven by the TrpR promoter acts as an inverter, while the
super-folder GFP (sfGFP) driven by the TetO promoter is the ultimate
readout. Also available in the registry are the two parts: BBa_K191004
and BBa_K191005.

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K892800 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K892800 parameters</partinfo>
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