View source for Restriction enzymes

Restriction enzymes work by recognizing a particular sequence of bases on the DNA. The enzyme then cuts the DNA's backbones, allowing the DNA to separate into two pieces.  <br>
For example, the enzyme EcoRI (see the figure) binds to the sequence GAATTC and cuts between the G and the A. It also cuts between the A and the G on the complementary strand. <br>
All parts in the Registry of Standard Parts are standardized by having "biobrick ends" composed of restriction sites in the order of EcoRI-NotI-Xba on the left side of the insert and SpeI-NotI-PstI on the right side.
*For the whole story on restriction enzymes, visit [https://parts.igem.org/cgi/htdocs/Assembly/standard_assembly.cgi here]
*For more information on the biobricks standard assembly, visit [https://parts.igem.org/cgi/htdocs/Assembly/index.cgi here]
*When faced with the task of analyzing long sequences of DNA for restriction sites, software is available to search DNA for recognition sequences.  One such useful program is [http://tools.neb.com/NEBcutter2/index.php NEB cutter], which interfaces the New England Biolab's library of restriction enzymes.