View source for Part:BBa K934012

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<partinfo>BBa_K934012 short</partinfo>

We constructed this part by combining [https://parts.igem.org/Part:BBa_K649000 BBa_K649000] and [https://parts.igem.org/Part:BBa_K081008 BBa_K081008].
This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.


[[Image:Plas-LuxI_result.png|thumb|center|500px|]]

To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI (BBa_K934012) with Ptrc-LasR to ''E.coli'' as “3OC12HSL dependent 3OC6HSL producer cell”. In this ''E.coli'', constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa_S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa_K395100]) to ''E.coli'' as a “Lux reporter cell”.

When the supernatants of the culture of “3OC12HSL dependent 3OC6HSL producer cell” were induced to “Lux reporter cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) is fuctioning.

We improved a previous part Plas-LuxI ([https://parts.igem.org/Part:BBa_K266000:Experience#User_Reviews BBa_K266000]) 
and accomplished a positive feedback system with our new part Plux-LasI ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). 


For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K934012 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K934012 parameters</partinfo>
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