View source for Part:BBa K777128

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<partinfo>BBa_K777128 short</partinfo>

[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [https://parts.igem.org/Part:BBa_J61002 J61002] and [https://parts.igem.org/Part:BBa_J23106 J23106]) and the plasmid backbone pSB1C3.


===Usage and Biology===
This construct can be useful in several ways, just like the original part [https://parts.igem.org/Part:BBa_J61002 J61002]. However, if you plan to send your parts in, they are already in the correct backbone, [https://parts.igem.org/Part:pSB1C3 pSB1C3].
# You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
# Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
# It can provide a nice selection system for inserting your basic parts into the pSB1C3 vector via the EcoRI/XbaI and PstI site. If you use a construct containing a strong promoter e.g. K777125, colonies containing a self-ligated plasmid after transformation will turn red and you can select for the non-red colonies. 
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* Here is the complete sequence as an [https://static.igem.org/mediawiki/2012/0/07/K777125-K777132.zip ApE file]
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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K777128 SequenceAndFeatures</partinfo>
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[[Image:K777128.jpg|frame|left|'''Fig. 2:''' Plasmid map of K777128]]

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===Functional Parameters===
<partinfo>BBa_K777128 parameters</partinfo>
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