View source for Part:BBa K632002

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<partinfo>BBa_K632002 short</partinfo>

The silicatein gene has been successfully fused to an existent ice nucleation protein (BBa_K265009). Gel electrophoresis and DNA sequencing both suggest the fusion product is the correct size, frame, and orientation. Silicatein functional assay was performed to verify the activity of silicatein, and the results indicate the silicatein is enzymatically active.

===Usage and Biology===
The INP-Silicatein fusion protein is a chimeric protein with the outer membrane associated domain (N-terminal domain) of ice nucleation protein fused to silicatein which is capable of polymerizing silicic acid monomers. The 2011 University of Minnesota iGEM team generated the following data regarding this part:

[[Image:inp-silicatein_standard_curve.png|480px]]
[[Image:inp-silicatein_results.png|480px]]
[[Image:inp-silicatein_vials.png|960px]]

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K632002 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K632002 parameters</partinfo>
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