View source for Part:pSB4A5:Experience

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
how you used this part and how it worked out.

===Applications of pSB4A5===

===User Reviews===
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<I>[[User:Rshetty|Reshma Shetty]]</I>
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[[Part:pSB4A5|pSB4A5]] has not been tested but is expected to be fully functional as a BioBrick standard vector.  When miniprepped [[Part:pSB4A5|pSB4A5]]-[[Part:BBa_I52002|I52002]] gives high miniprep yield similar to a high copy plasmids.
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<I>[[User:Swanson]]</I>
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'''***Warning:***'''[[Part:pSB4A5|pSB4A5]] does not appear to be correct in any distribution wells.  The [http://2011.igem.org/Team:Washington 2011 Washington iGEM team] sequenced pSB4A5 from distributions in the past several years and found it to be in fact pSB1A2 - a high-copy, not a low-copy, plasmid.  The UW iGEM team constructed pGA4A5, similar to pSB4A5 but for Gibson-friendly cloning.  For more information, visit [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Tookit] section of the Washington 2011 iGEM team's wiki.
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