View source for Part:BBa K523001

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<partinfo>BBa_K523001 short</partinfo>

This is the ''E. coli'' amylase gene ''malS''. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in <partinfo>BBa_K523000</partinfo>, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

===Usage and Biology===

While an amylase ought to be capable of degrading starch, the product protein is believed to be periplasmic and thus ought to only degrade starch if it leaks from the periplasm in significant quantities. Its natural function in ''E. coli'' presumably involves degrading shorter glucose chains: [http://dx.doi.org/10.1128/JB.00767-08 Lengsfeld ''et al'' (2008)] state that "MalS produces preferentially maltohexaose from longer maltodextrins in the periplasm".

The SignalP program predicts that a 17 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.

<font color="red">Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part <partinfo>BBa_K523006</partinfo>.</font>


<span class='h3bb'>'''Sequence and Features'''</span>
<partinfo>BBa_K523001 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K523001 parameters</partinfo>
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