Coding

Part:BBa_K4662031

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-06)


tracrRNA from S pyogenes

The natural S. pyogenes tracrRNA which is needed to be assembled scarless to an crRNA for efficent blocking of an target with the help of dCas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Part itself got not characterized but was successfully used in many assemblies.

e.g BBa_K4662069

[edit]
Categories
Parameters
None