Cell

Part:BBa_J72136

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-01-20)

JTK029: MC1061 upp::pir+FRT-kanR-FRT

Strain JTK029 is derived from MC1061 via integration of a pir expression cassette into the upp locus. The sequence contains the modified upp region and local genomic context.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2318
    Illegal XbaI site found at 3387
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4216
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2318
    Illegal XbaI site found at 3387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2318
    Illegal XbaI site found at 3387
    Illegal AgeI site found at 3682
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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