LOCUS       pSB1C3-P1010            2747 bp    DNA     circular UNA 08-DEC-2009
DEFINITION  Chloramphenicol Resistant plasmid for use in 3A selection assembly
            method.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      .
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      1857..1962
                     /note="Geneious name: T0 Terminator"
     CDS             complement(1977..2636)
                     /note="Geneious name: Cm R"
     primer_bind     2718..2741
                     /note="Geneious name: CMR fwd primer"
     primer_bind     complement(1856..1881)
                     /note="Geneious name: CmR rev primer"
     misc_feature    1..20
                     /note="Geneious type: primer"
                     /note="Geneious name: VF2"
     rep_origin      1091
                     /note="Geneious name: ORI"
     rep_origin      complement(1076..1690)
                     /note="Geneious name: rep (pMB1)"
     misc_feature    complement(972..991)
                     /note="Geneious type: primer"
                     /note="Geneious name: Verification reverse"
     misc_feature    915..934
                     /note="Geneious type: primer"
                     /note="Geneious name: pSB103 F"
     misc_feature    complement(50..69)
                     /note="Geneious type: primer"
                     /note="Geneious name: pSB103 R"
     stem_loop       complement(90..109)
                     /note="Geneious name: Stem-loop"
     misc_feature    complement(77..80)
                     /note="Geneious name: end of terminator"
     misc_feature    complement(140..816)
                     /note="Geneious name: ccd operon"
     -10_signal      complement(735..740)
                     /note="Geneious name: ccd -10"
     -35_signal      complement(758..763)
                     /note="Geneious name: ccd -35"
     CDS             complement(476..696)
                     /note="Geneious name: ccdA-"
     CDS             complement(169..474)
                     /note="Geneious name: ccdB"
     terminator      837..908
                     /note="Geneious name: E. coli his operon terminator"
     stem_loop       845..879
                     /note="Geneious name: Stem loop"
     misc_feature    133
                     /note="Geneious type: mutation"
                     /note="Geneious name: A to T mutation"
     variation       849..850
                     /note="Geneious name: Deletion of AC"
     misc_feature    1709..1725
                     /note="Geneious type: primer"
                     /note="Geneious name: Antibiotic-f"
     primer_bind     complement(1830..1849)
                     /note="Geneious name: pSB104.1XhoI-R"
     misc_feature    2734
                     /note="Geneious name: extra C discovered in sequencing"
     misc_feature    2052
                     /note="Geneious name: silent g-> a mutation discovered in
                     sequencing"
     misc_feature    7
                     /note="Geneious name: C->T mutation"
ORIGIN      
        1 tgccacttga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga
      121 attcgcggcc gcatctagag actggctgtg tataagggag cctgacattt atattcccca
      181 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc
      241 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc
      301 atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc
      361 actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc
      421 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc
      481 agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca
      541 tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca
      601 tggttgtgct taccagaccg gagatattga catcatatat gccttgagca actgatagct
      661 gtcgctgtca actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt
      721 cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac
      781 tgttatctgg cttttagtaa gccggatcca cgcgttacta gtagcggccg ctgcagtccg
      841 gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
      901 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1741 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1801 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
     1861 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1921 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     1981 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     2041 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     2101 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     2161 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     2221 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     2281 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     2341 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     2401 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     2461 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     2521 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     2581 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     2641 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2701 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgag
//
