Talk:Part:BBa I13453:Experience
Greetings jkm. Just a suggestion... It would be very helpful if you could annotate the Cap protein and AraC protein binding sites. Lee et al, PNAS 1981 had identified these binding sites via DNA footprinting analyses. On the I13453 sequence, the Cap protein binds to 10-35 on the plus strand and 1-30 on the minus strand. I have not looked at the others very closely (B-site/ AraC binding site and the polymerase binding site).