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  • Part:BBa K302033
    ...of both genes is turned off (as both are under contol of same promoter) ''mazE'' will be degraded faster than ''mazF''. There is then no inhibiton of ''ma ...NA binding site on the second subunit of MazF[3]. Thus, in the presence of MazE, MazF cannot bind to or cleave substrate RNA.
    21 KB (3,117 words) - 18:26, 13 October 2022
  • Part:BBa K4604023
    ...ink" href="https://parts.igem.org/Part:BBa_K2308010">BBa_K2308010</a>), <i>mazE</i> (antitoxin, <a class="link" href="https://parts.igem.org/Part:BBa_K4604 ...enes: an antibiotics resistance used as selection marker, an antitoxin (<i>mazE</i>, <a href="https://parts.igem.org/Part:BBa_K4604037">BBa_K4604037</a>) u
    35 KB (5,319 words) - 14:18, 12 October 2023
  • Part:BBa K1937009:Design
    ...3). The part was designed to be carried on a plasmid while its counterpart MazE/Zeta was carried on another. Unfortunately, only Epsilon/MazF part was succ ...a strong RBS. A theophylline-dependent riboswitch was used to prevent the MazE toxin expression during the cloning steps in presence of theophylline (Topp
    4 KB (547 words) - 17:47, 18 October 2016
  • Part:BBa K1937010:Design
    ...3). The part was designed to be carried on a plasmid while its counterpart MazE/Zeta was carried on another. Unfortunately, only Epsilon/MazF part was succ ...a strong RBS. A theophylline-dependent riboswitch was used to prevent the MazE toxin expression during the cloning steps in presence of theophylline (Topp
    11 KB (649 words) - 17:52, 18 October 2016
  • Part:BBa K4656010
    ...rge number of toxic proteins, resulting in the death of E. coli. (MazF and MazE belong to the toxin-antitoxin suicide system.) ...refore, the expression of toxin MazF in the pathway was inhibited, and the MazE antitoxin protein at the end of the gene route was expressed instead. Event
    3 KB (399 words) - 12:40, 17 September 2023
  • Part:BBa K4211015:Design
    <br>[2] Zhou, Y., Kong, D., Wang, X. et al. A small and highly sensitive red/far-red optogenetic switch for app ...RNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis. Mol Cell 52, 447-458 (2013). https://doi.org:10.1016/
    2 KB (216 words) - 07:50, 6 October 2022