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  • Part:BBa K4245006:Experience
    [[File:RCA figure 9.png|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA-1 RCP product. </i>]] ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t
    10 KB (1,573 words) - 12:39, 12 October 2023
  • Part:BBa K4245100:Experience
    [[File:RCA figure 9.png|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA-1 RCP product. </i>]] ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t
    10 KB (1,565 words) - 12:13, 12 October 2023
  • Part:BBa K4245107:Experience
    [[File:RCA figure 9.png|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA-1 RCP product. </i>]] ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t
    10 KB (1,567 words) - 12:42, 12 October 2023
  • Part:BBa K4245009:Experience
    [[File:RCA 133a Gel.png|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA 133a RCP product.RCP is visible in wells 7 and 8.</i>]] ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t
    5 KB (893 words) - 13:00, 12 October 2023
  • Part:BBa K4245200:Experience
    [[File:RCA figure 9.png|thumb|center|500px|<i>Figure 1. Image of gel ran with miRNA-1 RCP product. </i>]] ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t
    8 KB (1,309 words) - 13:00, 12 October 2023
  • Part:BBa J23119
    ...2018 Hawaii iGEM team evaluated the 40 most frequently used BioBricks and ran them through an internal priming screening process that we developed using <p>Both comprehensive literature exploration (Wang, 2019) and our empirical investigations confirm its capacity to drive prote
    22 KB (3,386 words) - 15:59, 12 October 2023
  • Part:BBa K4959003
    ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify
    8 KB (1,200 words) - 15:05, 10 October 2023
  • Part:BBa K4959004
    ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify
    8 KB (1,130 words) - 11:19, 11 October 2023
  • Part:BBa K4959005
    ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify ...ds using two restriction endonucleases, Nde1 and Xho1. After digestion, we ran the gel by agarose gel electrophoresis, followed by gel recovery to purify
    8 KB (1,200 words) - 11:19, 11 October 2023
  • Part:BBa K1077002
    ...having more plasmids in the ON state, producing protein. Unfortunately, we ran out of time before we could perform the digest assay on the cultures. The r ...rprising amount of stability as indicated by the lack of amilCP. Again, we ran out of time to perform the digest assay on the cultures.
    16 KB (2,690 words) - 16:04, 29 September 2013
  • Part:BBa K3168000
    ...ease is used a lot in research to facilitate efficient genome engineering (Ran, 2013). Cas9 can target any sequence by simply specifying a 20 nucleotide t Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimic
    3 KB (393 words) - 15:17, 21 October 2019
  • Part:BBa K3168001
    ...ease is used a lot in research to facilitate efficient genome engineering (Ran, 2013). Cas9 can target any sequence by simply specifying a 20 nucleotide t Park, J. J., Dempewolf, E., Zhang, W., & Wang, Z. Y. (2017). RNA-guided transcriptional activation via CRISPR/dCas9 mimic
    3 KB (442 words) - 15:19, 21 October 2019
  • Part:BBa K4683003:Experience
    ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (
    4 KB (596 words) - 11:42, 12 October 2023
  • Part:BBa K4683004:Experience
    ...o detect specific miRNA, and therefore be applicable for serum testing, we ran RCA using the hsa-miR-1-3p padlock <partinfo>BBa_K4245200 </partinfo> in t alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (
    4 KB (596 words) - 02:38, 12 October 2023
  • Part:BBa C0051
    ...2018 Hawaii iGEM team evaluated the 40 most frequently used BioBricks and ran them through an internal priming screening process that we developed using <p >Cheng L, Wang J, Zhao X, et al. An antiphage Escherichia coli mutant for higher productio
    28 KB (4,107 words) - 15:02, 12 October 2023
  • Part:BBa M36344
    ...from Wang, J., De Luca, V. 2005. We then took the amino acid sequence and ran it through OPTIMIZER, referenced below, to arrive at our nucleotide sequenc Wang, J., De Luca, V. “The biosynthesis and regulation of biosynthesis of Conc
    2 KB (352 words) - 21:27, 4 May 2014
  • Part:BBa K3407006
    ...r. Urea-PAGE 15% of 19:1 (Acrylamide:Bisacrylamide) ratio. Electrophoresis ran for ~3 hours at 120 V.]] </li> Wang, X., Xu, X., Ma, Z., Huo, Y., Xiao, Z., Li, Y. and Wang, Y., 2020. Dynamic Mechanisms For Pre-Mirna Binding And Export By Exportin-
    21 KB (3,233 words) - 11:16, 27 October 2020
  • Part:BBa K3407022
    ...r. Urea-PAGE 15% of 19:1 (Acrylamide:Bisacrylamide) ratio. Electrophoresis ran for ~3 hours at 120 V.]] </li> Wang, X., Xu, X., Ma, Z., Huo, Y., Xiao, Z., Li, Y. and Wang, Y., 2020. Dynamic Mechanisms For Pre-Mirna Binding And Export By Exportin-
    21 KB (3,235 words) - 03:18, 28 October 2020
  • Part:BBa K3924032
    ...the proteins were affinity-purified using Ni-NTA or Co-NTA beads. Then we ran the SDS-PAGE gel to Coomassie brilliant blue staining and Western Blotting. [2] Wang, P., Tang, W., Li, Z., Zou, Z., Zhou, Y., Li, R., Xiong, T., Wang, J., & Zou, P. (2019). Mapping spatial transcriptome with light-activated p
    7 KB (989 words) - 17:49, 21 October 2021
  • Part:BBa K4130000
    We first ran a control experiment to examine the effect of addition of the beads themsel *<p align="justify"> [2] Sandt, C. H., Y. D. Wang, R. A. Wilson, and C. W. Hill. 1997. Escherichia coli strains with nonimmun
    28 KB (4,120 words) - 23:56, 13 October 2022

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