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  • Part:BBa K638402
    This TorA leader sequence variant has been successfully used to export GFP to the per ...ld-type MC4100AR cells (top) and the ΔtatC strain (bottom) containing the torA–GFP fusion plasmid pJDT1 were grown in the presence of arabinose, then re
    6 KB (874 words) - 00:51, 22 September 2011
  • Part:BBa K1012002
    DNA encoding the TorA twin-arginine signal peptide from <i>E. coli</i> TMAO reductase. This will ...plasm. Indeed, it was this unusual order of events, which would imply that TorA must be exported in a folded form, that led to the hypothesis that a transp
    6 KB (934 words) - 14:30, 26 October 2013
  • Part:BBa K1978000
    ...e linked to BtuF, a protein capable of binding vitamin B<sub>12</sub>. The TorA signal peptide allows export of fully-folded proteins through the inner mem ...n-arginine motif which is vital for the recognition by the Tat system. The TorA signal sequence and the sequence coding for BtuF are connected by a linker
    8 KB (1,220 words) - 17:20, 20 October 2016
  • Part:BBa K3016100
    ...cation (Tat) pathway. Derived from Vibrio natriegens’ TMAO reductase (<i>torA</i>) gene. Note: TorA signal peptide may be prone to inclusion body formation in <i>Escherichia c
    4 KB (598 words) - 18:05, 21 October 2019
  • Part:BBa K3016200
    This is a composite part containing <i>Vibrio natriegens'</i> TorA Tat signal peptide ([https://parts.igem.org/Part:BBa_K3016100 BBa_K3016100] ...cation (Tat) pathway. Derived from Vibrio natriegens’ TMAO reductase (<i>torA</i>) gene.
    5 KB (697 words) - 18:03, 21 October 2019
  • Part:BBa K797002
    This part is coding N-terminal torA signal region. Proteins conjugated torA signal are recognized by Tat secretion pathway and they are secreted from c ...assembly. For these reasons, all of other teams make an effort to combine TorA signal to targets, such as using Gibson assembly. That's too trouble!<br>
    2 KB (341 words) - 03:58, 27 September 2012
  • Part:BBa K2306001
    TU Delft 2017 designed a basic part encoding the TorA-GFP sequence [https://parts.igem.org/Part:BBa_K2306000 (BBa_K2306000)], and For characterization, TorA-GFP was transformed into both the E.coli BW25133 strain E. coli BW25133 st
    10 KB (1,537 words) - 20:16, 31 October 2017
  • Part:BBa K2244003
    This part is a coding sequence (opdA) with a TorA signal peptide fused to its N-terminus for protein export to periplasmic sp ...rginine signal peptide of E. coli trimethylamine N-oxide (TMAO) reductase (TorA), was added to the N-terminal of the opdA. TMAO reductase is a periplasmic
    8 KB (1,117 words) - 05:08, 19 October 2021
  • Part:BBa K3016100:Experience
    ...3016600 BBa_K3016600]), a slow-bleaching GFP variant, creating a composite TorA-YGFP part ([https://parts.igem.org/Part:BBa_K3016200 BBa_K3016200]) to test ...s.png|thumb|530px|center|<font size="1">Vibrio natriegens cells expressing TorA-YGFP</font>]]
    3 KB (466 words) - 18:42, 21 October 2019
  • Part:BBa K3016600
    ...ps://parts.igem.org/Part:BBa_K3016100 BBa_K3016100]), creating a composite TorA-YGFP part ([https://parts.igem.org/Part:BBa_K3016200 BBa_K3016200]), to tes ...s.png|thumb|530px|center|<font size="1">Vibrio natriegens cells expressing TorA-YGFP</font>]]
    4 KB (531 words) - 18:07, 21 October 2019
  • Part:BBa K3016600:Experience
    ...ps://parts.igem.org/Part:BBa_K3016100 BBa_K3016100]), creating a composite TorA-YGFP part ([https://parts.igem.org/Part:BBa_K3016200 BBa_K3016200]), to tes ...s.png|thumb|530px|center|<font size="1">Vibrio natriegens cells expressing TorA-YGFP</font>]]
    3 KB (512 words) - 18:45, 21 October 2019
  • Part:BBa K3016200:Experience
    ...s.png|thumb|530px|center|<font size="1">Vibrio natriegens cells expressing TorA-YGFP</font>]] ...|530px|center|<font size="1"><i>Escherichia coli</i> DH5a cells expressing TorA-YGFP</font>]]
    3 KB (451 words) - 18:40, 21 October 2019
  • Part:BBa K1202115:Experience
    ===Signal Peptide experiments, TorA=== ...is construct secrete GFP successfully, that means signal peptide works and TorA can be used in Apoptin or E4orf4 export.
    4 KB (555 words) - 11:19, 9 October 2013
  • Part:BBa K1978001
    ...ce linked to GlmS, protein capable of binding vitamin B<sub>12</sub>. The TorA signal peptide allows export of fully-folded proteins through the inner mem ...n-arginine motif which is vital for the recognition by the Tat system. The TorA signal sequence and the sequence coding for BtuF are connected by a linker
    7 KB (1,055 words) - 17:24, 20 October 2016
  • Part:BBa K1012001
    ...dy. As shown in Figure 1A, PP1 was successfully produced when fused to the TorA and MalE signal peptides. ...<i>E. coli</i> MC4100 cells producing PP1 fused to the signal peptides of TorA (TorASP-PP1; Tat-targeting) or MalE (MalESP-PP1; Sec-targeting) or <b>(B)</
    14 KB (2,216 words) - 15:08, 26 October 2013
  • Part:BBa K2717013:Experience
    ...tor containing only VHb. Experiments have shown that the strain expressing TorA and VHb has a higher environmental capacity and a faster growth rate than t ...-Stbl3. We also design another Stbl3 with plasmid contains VHb but without TorA signal peptide as control.
    3 KB (444 words) - 22:05, 17 October 2018
  • Part:BBa K2830015
    TorA is a signal peptide that can transport proteins from gram-negative bacteria ...the cytoplasmic membrane. the TatBC complex recognizes the signal sequence TorA and the folded protein is translocated via TatA into periplasm
    2 KB (277 words) - 21:10, 17 October 2018
  • Part:BBa K2830016
    TorA is a signal peptide that can transport proteins from gram-negative bacteria ...the cytoplasmic membrane. the TatBC complex recognizes the signal sequence TorA and the folded protein is translocated via TatA into periplasm
    2 KB (277 words) - 21:16, 17 October 2018
  • Part:BBa K3924016
    Name: TorA<br/> TorA is one of candidate secretion peptides we screened out, which is a most ess
    7 KB (969 words) - 20:54, 21 October 2021
  • Part:BBa K4738013
    ...ively produces a TorA-PhaP-His6 fusion protein, which secretes through the TorA Type 2 twin-arginine translocation (Tat) bacterial secretion pathway. The H ...this part to verify the secretion of TorA-tagged phasin from E. coli. The TorA (trimethylamine N-oxide reductase A) system is beneficial since it favors f
    3 KB (393 words) - 15:34, 12 October 2023

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