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  • ...w part BBa_K4411032, which is composed of casposase, TIR DNA sequence, and TSD DNA fragment. ...se, BBa_K4411032) homologous to the Cas1 endonuclease, which can recognize TSD and TIR elements to implement gene insertion and develop an in vitro gene e
    8 KB (1,174 words) - 14:19, 12 October 2022
  • ...inase (casposase) homologous to the Cas1 endonuclease, which can recognize TSD and TIR elements to implement gene insertion and develop an in vitro gene e ...hether the long fragment gene with TIR sequence could be inserted into the TSD sequence effectively and correctly, the protein casposase was added for rea
    8 KB (1,142 words) - 08:51, 26 September 2022
  • ...erties: a composite part composite by pUC19 plasmid backbone and C Target1-TSD-C Target2 DNA sequence ...lasmid with Ampicillin resistance. Then we did PCR to insert the C Target1-TSD-C Target2 DNA fragment by designing recombinant primers according to the ta
    3 KB (473 words) - 07:26, 26 September 2022
  • ...x with it. Then the TIR containing DNA fragment would be inserted into the TSD sequence and complete the gene editing [1-5]. ...hether the long fragment gene with TIR sequence could be inserted into the TSD sequence effectively and correctly, the protein casposase was added for rea
    5 KB (797 words) - 08:11, 26 September 2022
  • ...rties: a composite part composite by pET28a plasmid backbone and C Target1-TSD-C Target2 DNA sequence ...t1-TSD-C Target2 DNA sequence [1-5]. In this part, we insert the C Target1-TSD-C Target2 DNA fragment by designing recombinant primers according to the ta
    3 KB (379 words) - 07:33, 26 September 2022
  • ...x with it. Then the TIR containing DNA fragment would be inserted into the TSD sequence and complete the gene editing [1-5]. ...hether the long fragment gene with TIR sequence could be inserted into the TSD sequence effectively and correctly, the protein casposase was added for rea
    5 KB (665 words) - 08:28, 26 September 2022
  • <I>TSD</I>
    2 KB (257 words) - 16:41, 27 October 2016
  • TSD
    386 B (52 words) - 06:54, 2 September 2022
  • C Target1-TSD-C Target2
    406 B (55 words) - 07:00, 2 September 2022