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- ...or sp), and the controlled group was transfected with null sponge plasmid (sponge-empty-pcDNA3.1-P2A-C, or spe), the ratio of these two plasmids was from 1:1 ...N-eGFP, sponge refers to sponge-pcDNA3.1-P2A-C, and sponge empty refers to sponge-empty-pcDNA3.1-P2A-C. )4 KB (598 words) - 13:38, 21 October 2021
- ...o compete miRNAs [4]. This mechanism gives rise to our idea about fusing a sponge RNA based on the sequences of lncRNA MALAT1 with binding sites complementar ...e to the miR-22 concentration in cells. The part is called Improved miR-22-sponge-pepper (BBa_K5082017) and its sequence is shown in Figure 4.8 KB (1,145 words) - 11:46, 28 September 2024
- ...onitor the expression of miR-21 in cells. And the plasmid of pSB1C3-miR-21-sponge-4s will be submitted to 2018IGEM. ...ormula.Finally,We find that miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge6s.2 KB (401 words) - 12:14, 9 October 2018
- ...A binding sites (MBS); (2) a central bulge to avoid the degradation of the sponge, this would be realized by the addition, elimination or substitution of som ...between the sponge and miRNA, the bulge was at the central position of the sponge.<br/>2 KB (362 words) - 09:52, 27 September 2018
- ...a miRNA of interest, is used to be a inhibitor of miRNA. We proposed miRNA sponge could be a monitor to reflect the change of miRNA expression in cells. ...ct the expression of miR-21, 293T cells were transfected with pEGFP-miR-21-sponge-4s with different concentration of pHAGE-pre-miR-21. We used the plasmid of2 KB (306 words) - 13:48, 5 October 2018
- ...gested miR-21-sponge-4s is more sensitive than miR-21-sponge-2s and miR-21-sponge-6s. We synthesized miR-21-sponge-4s from BBa_K2514000.2 KB (275 words) - 12:16, 11 October 2018
- ==The effect of pEGFP-miR-155-sponge-1 as a monitor to detect the expression of miR-155== ...with controls (Fig. 7). Taken together, these results reveal pEGFP-miR-155-sponge-1 could act as a monitor to detect the expression of miR-155.3 KB (420 words) - 04:10, 4 October 2020
- ...to compete miRNAs[2]. This mechanism gives rise to our idea about fusing a sponge RNA based on the sequences of lncRNA MALAT1 with binding sites complementar Fig 2. The diagram of miR-22-sponge-pepper6 KB (852 words) - 11:36, 9 October 2023
- ...of interest, which inhibit miRNA activity [1]. When perfectly matched, RNA sponge site will bind to the target miRNA preventing it from binding to their targ ...of miR-155 in cells.the GFP value of cells transfected with pEGFP-miR-155-sponge-1 plasmid could show the different expression of miRNAs in cells.What we ex3 KB (448 words) - 02:02, 26 October 2020
- ...to compete miRNAs[2]. This mechanism gives rise to our idea about fusing a sponge RNA based on the sequences of lncRNA MALAT1 with binding sites complementar Fig 2. The diagram of miR-145-sponge-pepper6 KB (822 words) - 11:39, 9 October 2023
- ===effect of pEGFP-miR-155-sponge-1 in breast cancer cells=== ...he results suggested the GFP value of cells transfected with pEGFP-miR-155-sponge-1 could show the different expression of miRNAs in cells.2 KB (289 words) - 15:13, 3 October 2020
- ...iR-21 binding sites with 3-nt spacers for bulged sites. Another one mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We w ...microscopy. The fluorescence of GFP in cells transfect with pEGFP-miRNA-21-sponge-6s was lower in that of controls. The result confirmed that the fluorescenc5 KB (674 words) - 02:29, 12 October 2022
- The transcription of this miRNA sponge sequence will allow you to combine the free hsa-miR-199a,hsa-miR-195 and hs ...llowing figures, spe stands for sponge-empty, which is the plasmid without sponge sequences, as the control group. <br>3 KB (436 words) - 17:44, 21 October 2021
- The miRNAsong website[2] was used to design the basic sponge framework, the free energy cutoff was 25Kcal/mol, and the miss was checked ...b-sponge can efficiently inhibit the endogenous miR148b compared to miR141-sponge when driven by the TNBC-specific promoter s(ESR1)p.</center><br/><br/>3 KB (467 words) - 19:34, 21 October 2019
- ...iR-21 binding sites with 3-nt spacers for bulged sites. Another one mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We wa2 KB (262 words) - 08:49, 20 October 2017
- <b>Fig. 1</b> The sequence of miRNA sponge. The sponge is composed of several complementary tandem repeats of each miRNA flanking2 KB (273 words) - 13:37, 21 October 2021
- ...s transcribed. Oure RNA sponde can be cotransfected simultaneously. If the sponge binds to the target miRNA and initiates the knock down, the fused Renilla l ...iferase® Reporter 1000 Assay System. It proves that our pCDH-linear mir21 sponge knocks down the level of miR21.<br>5 KB (700 words) - 13:23, 20 September 2015
- ...ug regularly for cancer treatment. For our system to work efficiently, the sponge plasmid is applied together with the repressilator to smooth out the observ ...atory expression of our reporter genes for 12 hours, respectively with the sponge plasmids pLPT145 and pLPT41.4 KB (545 words) - 18:20, 27 October 2020
- ...lls [2]. Additionally, it has been reported that MALAT1 can also act as a "sponge" for miR-429, thereby regulating the migration, apoptosis, and other metabo https://static.igem.wiki/teams/4789/wiki/sponge.png4 KB (481 words) - 07:42, 6 October 2023
- ...lls [2]. Additionally, it has been reported that MALAT1 can also act as a "sponge" for miR-429, thereby regulating the migration, apoptosis, and other metabo https://static.igem.wiki/teams/4789/wiki/sponge.png3 KB (428 words) - 07:54, 6 October 2023