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- ...our samples. Our negative control contained the transformed E. coli and EZ rich defined media, but did not contain any TMAO. All samples were initially pre ...our samples. Our negative control contained the transformed E. coli and EZ rich defined media, but did not contain any TMAO. All samples were initially pre11 KB (1,902 words) - 22:07, 12 June 2018
- ...dings, the LldPRD promoter does not function under both anoxic and glucose-rich environment, which are the main characteristics of solid breast cancer [36] ...A promoter. If high lactate concentration is sensed in hypoxic and glucose-rich environment, then binding of L-lactate to lldR changes its conformation so59 KB (8,345 words) - 09:22, 2 October 2024
- ...this part and requested the plasmid from them.We planned to insert an A/U rich region in the upstream of the RBS of [https://parts.igem.org/wiki/index.php ===The mechanism of A/U-rich 5'-UTR to promote protein synthesis===4 KB (565 words) - 01:26, 22 October 2019
- 1. H-rich ferritins turn over more rapidly than L-rich ferritins 2. H-rich ferritins accumulate in iron-rich tissues8 KB (1,127 words) - 02:09, 21 October 2020
- 1. H-rich ferritins turn over more rapidly than L-rich ferritins 2. H-rich ferritins accumulate in iron-rich tissues10 KB (1,432 words) - 13:43, 13 October 2022
- ...hed. After making all of the acidic solutions, we began again with pure EZ-Rich media and added sodium hydroxide (NaOH) dropwise to create solutions with t ...lls, and vacuumed off the supernatant. We then resuspended the cells in EZ-Rich media, such that each aliquot had a final OD600 of 0.2. We then performed a9 KB (1,516 words) - 09:55, 13 December 2017
- ...ies: Colicin E1 expression induced by sodium lactate in anoxia and glucose rich environment <br>Design: codon optimized LldRPD promoter for glucose-rich and anoxic environment17 KB (2,651 words) - 15:51, 12 October 2023
- ...which can be utilized as a signal reporter. The conformation of released G-rich oligonucleotides would change into G-quadruplex DNA with the presence of 2. ...ACGTACCAAC (complementary to some parts of hairpin siRNA, Fig.3) and the G-rich DNA: '''AATACGAC'''GGG'''CTAT'''GGGTTTTGGGTTTTGGGAGCTA. Then, we put them t7 KB (1,120 words) - 18:38, 21 October 2019
- ...which can be utilized as a signal reporter. The conformation of released G-rich oligonucleotides would change into G-quadruplex DNA with the presence of 2. This G-rich DNA duplex was designed to test the change of siRNA named 29128. For more d5 KB (678 words) - 00:19, 19 October 2019
- ...4). Hence, a ribosome bound at the RBS would be anticipated to protect A/U-rich regions flanking the start codon from RNase E cleavage. In support of this ...2005) provided further support for these findings. The introduction of A/U-rich sequences upstream of the SD increased translation and the stability of lac22 KB (3,197 words) - 13:52, 19 October 2021
- <p>We assessed the cytotoxic potential of azurin-rich bacterial lysates on p53 (+) and p53 (-) cell lines. Literature suggests, t ...from HeLa CCL-2 lc cell cultures incubated with different amount of azurin-rich lysate. Decrease in citrine fluorescence is only detected in samples were c12 KB (1,736 words) - 03:29, 2 November 2017
- ...another dynamic range experiment by culturing the bacteria overnight in EZ-Rich Medium without glucose and transferring them to higher glucose concentratio ...ith each concentration doubling the previous. Bacteria were cultured in EZ-Rich Medium without glucose. Mean ± standard error shown for each point."]]6 KB (904 words) - 00:55, 12 December 2018
- ...ins, translational factors, or nucleoid proteins, is accompanied by an A/U-rich region upstream of the translation initiation codon and the ribosome-bindin Fig.1. The mechanism of A/U-rich 5'-UTR to promote protein synthesis.<BR>4 KB (671 words) - 23:56, 21 October 2019
- ...ins, translational factors, or nucleoid proteins, is accompanied by an A/U-rich region upstream of the translation initiation codon and the ribosome-bindin We planned to insert an A/U rich region in the upstream of the RBS of [https://parts.igem.org/wiki/index.php4 KB (603 words) - 02:38, 22 October 2019
- ...iginal Great Bay part was modified at 6 bases to make the sequence less GC-rich and reduce the number of base repeats, thus making it easier to be synthesi ...between poly-alanine regions and hydrophilic interactions between glycine-rich regions confer the strength and elasticity of dragline silk respectively [36 KB (912 words) - 13:27, 12 October 2022
- ...iginal Great Bay part was modified at 6 bases to make the sequence less GC-rich and reduce the number of base repeats, thus making it easier to be synthesi ...between poly-alanine regions and hydrophilic interactions between glycine-rich regions confer the strength and elasticity of dragline silk respectively [36 KB (897 words) - 13:26, 12 October 2022
- ...otein consisting of four repetitive poly-alanine regions linked by glycine rich regions and fused to a C-terminal domain, all from the African nursery web ...between poly-alanine regions and hydrophilic interactions between glycine-rich regions confer the strength and elasticity of dragline silk respectively [25 KB (756 words) - 13:15, 12 October 2022
- ...otein consisting of four repetitive poly-alanine regions linked by glycine rich regions and fused to <partinfo>BBa_K3264002</partinfo>. ...between poly-alanine regions and hydrophilic interactions between glycine-rich regions confer the strength and elasticity of dragline silk respectively [35 KB (778 words) - 13:16, 12 October 2022
- ...'' instead of sodium thioglycolate, a mixture called '''<font color=red>En-rich MSGM</font>'''. In <<''Effects of growth medium composition, iron sources a <font size=3>'''En-rich MSGM's ingredients'''8 KB (1,090 words) - 07:28, 9 October 2011
- This composite device produces and '''exports''' a '''Histidine rich''' peptide, that is '''import enhanced''' (thanks to a trojan peptide). * '''''Payload''''': this is the exported '''Histidine''' rich domain of the protein ''(a His-Tag)''3 KB (388 words) - 20:27, 27 October 2012