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  • Part:BBa K986000
    ...he polyphosphatase then degrades phosphates present when there is too much phosphorus in water. We use it as a CDS in our project. ...at position 719, and this mutant significantly enhances the efficiency of phosphorus uptake by ''E. coli'' from the environment.''' Here are our experimental in
    12 KB (1,857 words) - 16:02, 11 October 2022
  • Part:BBa K4252002
    For all we know, phosphorus is the key substance that causes eutrophication in water. It will not only ...ore phosphorus was characterized indirectly by measuring the difference in phosphorus concentration within the bacterial medium at the same growth density of the
    11 KB (1,763 words) - 15:55, 11 October 2022
  • Part:BBa K4252002:Experience
    ...tore phosphorus is characterized indirectly by measuring the difference in phosphorus concentration within the bacterial medium at the same growth density of the ...medium, continued to incubate (37°C, 225 rpm) and the growth density and phosphorus concentration were measured hourly starting from 4 h after resuspension.<br
    11 KB (1,660 words) - 07:41, 11 October 2022
  • Part:BBa K2587009
    ...tart metabolizing phosphite, also known as phosphonic acid, an alternative phosphorus source not commonly metabolizable by most organisms. In our three-way co-culture, we want to use phosphite as a non-metabolizable phosphorus source. Only our engineered <i>S.cerevisiae</i> strain is able to convert i
    12 KB (1,966 words) - 23:49, 17 October 2018
  • Part:BBa K3408007
    ①Drawing the standard curve of inorganic phosphorus ...s content as ordinate (take 0.05 mL of the diluent above and the inorganic phosphorus content is: 0.00, 0.16, 0.32, 0.48, 0.64, 0.8 μmol respectively) and absor
    13 KB (1,657 words) - 03:07, 28 October 2020
  • Part:BBa K1807002
    ...> using medium with normal phosphorus concentration and medium with double phosphorus concentration. ...high phosphorus uptake both in normal medium and in medium with twice the phosphorus concentration.</p>
    4 KB (644 words) - 03:06, 22 October 2021
  • Part:BBa K2789020
    ...Poly P) thus use the natural phosphorus transport system to accumulate the phosphorus in the bacteria.<br/> ...600 about 1.6). The supernatant is diluted with 99 times water to make the phosphorus suitable to measure. (3 same groups are done in the test. pET28a(+) is a ex
    3 KB (533 words) - 08:36, 17 October 2018
  • Part:BBa K5034210
    ...effects of the introduction of this element on electricity production and phosphorus metabolism. ...apacity after the introduction of <i>PPX</i> gene.(Fig.3) In addition, the phosphorus accumulation capacity also decreased compared with WT.(Fig.4)
    6 KB (819 words) - 02:44, 2 October 2024
  • Part:BBa K5034217
    ...as able to improve the efficiency of electrical production and improve the phosphorus accumulation capacity of <i>S.oneidensis</i>. ...sferring into the <i>S.oneidensis</i>, both the electricity production and phosphorus accumulation efficiency were significantly improved compared to the wild ty
    10 KB (1,482 words) - 11:53, 2 October 2024
  • Part:BBa K2325102
    ...een constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli. ...sub>3</sub>•5H<sub>2</sub>0 (1.32mM) and NH<sub>4</sub>Cl serving as the phosphorus and nitrogen sources, respectively.]]
    2 KB (255 words) - 13:30, 29 October 2017
  • Part:BBa K4252001
    As a phosphorus export element. ...s still unclear. But based on experimental phenomena we choose YjbB as the phosphorus output element in our project.
    3 KB (390 words) - 11:00, 11 October 2022
  • Part:BBa K5034229
    ...apacity after the introduction of <i>PPX</i> gene.(Fig.6) In addition, the phosphorus accumulation capacity also decreased compared with WT.(Fig.7) <p style="text-align: center;">Figure 7: Statistical data on the phosphorus accumulation capacity of <i>S. oneidensis</i> with <i>PPX</i></p>
    7 KB (1,004 words) - 02:44, 2 October 2024
  • Part:BBa K5034223
    ...as able to improve the efficiency of electrical production and improve the phosphorus accumulation capacity of ''S.oneidensis''. ...ficiency were significantly improved compared to the wild type(Fig.5). The phosphorus accumulation effect was measured in M9 cultural medium, and the electricity
    7 KB (1,060 words) - 04:50, 2 October 2024
  • Part:BBa K2325001
    ...een constructed into expression vectors to modify the metabolic pathway of phosphorus in E.coli. ...combination of CFPPK and KPD can force the bacteria to constantly remain "phosphorus starvation" state, thus pushing them to continuously oxidize phosphite.
    4 KB (577 words) - 13:37, 11 October 2022
  • Part:BBa K2789021
    ...release" step must be complete and thorough.PPN can help PPX to co-release phosphorus (improved about 10 times in speed). And we created PPN part this year and w ...darker than the ppx and ppn which means the ppn and ppx strain have lower phosphorus concentration. The photo is taken with same parameter at 1000×.Each group
    3 KB (533 words) - 03:09, 18 October 2018
  • Part:BBa K2789032
    ...the ends of Poly P, this two protein together can dramatically release the phosphorus from the bacteria. This two proteins play the opposite effect from PPK(Poly ...e most bacteria. The supernatant is diluted with 49times water to make the phosphorus suitable to measure. The reaction result shows below:<br/>
    3 KB (530 words) - 08:48, 17 October 2018
  • Part:BBa K5449005
    ...al impacts and preserves water quality, while allowing for the recovery of phosphorus for reuse as a nutrient in farming. ...to capture and recycle phosphate from municipal wastewater, ensuring that phosphorus does not contribute to pollution while simultaneously providing an opportun
    20 KB (2,654 words) - 07:16, 2 October 2024
  • Part:BBa K5034214
    ...sis.</i> to develop the best ability to produce electricity and polymerize phosphorus. .... We found that <i>SPK3</i> with RBS BBa-B0031 has the lowest capacity for phosphorus polymerization but the highest electroproduction capability compared to oth
    7 KB (1,037 words) - 08:19, 2 October 2024
  • Part:BBa K4252009
    ...e transport and others like phoA, phoE, phoP to control the other forms of phosphorus. <br /> ...en from the supernatant, and diluted ten times for subsequent detection of phosphorus concentration.
    12 KB (1,867 words) - 13:39, 11 October 2022
  • Part:BBa K4252009:Experience
    ...en from the supernatant, and diluted ten times for subsequent detection of phosphorus concentration. #Repeat step 3 until the bacterium no longer grows and the phosphorus concentration value detected subsequently does not change.
    9 KB (1,391 words) - 13:44, 11 October 2022

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