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- ...enome DNA of this engineered phage, shown in Fig. 1, needs two functions. One is inducible expression of <i>g2p</i>. We thus designed to replace the pro ...ith two modifications related to our design kept plaque forming activity. One is replacement of the promoter for <i>g2p</i> with a constitutive promoter,6 KB (937 words) - 19:48, 27 October 2013
- ...ated paramagnetic microbeads in solution. The biotin-streptavidin bond, is one of the strongest found in nature, thus further confirming the stability and ...t et al.). This subsequently means that if we induced the cells to swim in one direction, there would be no guarantee with which side they would swim. Bec27 KB (4,191 words) - 02:51, 31 October 2017
- ...ced by some splicesome. Alternative splicing can produce two isoforms from one gene, thus can be used in our project to monitor whether the Sic1 system wo ...’ sites as well as the upstream 5’ sites with preference to the former one.</p>27 KB (4,233 words) - 05:09, 20 October 2021
- ...rt had to be ordered in two parts since it could not be synthesized in one piece. Therefore, Gibson assembly was the first step in the assembly of the part. ...PCR were designed to bind to the backbone on either side of the insertion piece and the amplified region has a length of 2004 bp.22 KB (3,444 words) - 15:56, 12 October 2023
- ...since the band of Sortase A5M is a little higher than the one of Sortase A7M. ...ission maximum of TAMRA over time is also visible in the negative control. One106 KB (11,833 words) - 14:02, 19 October 2021
- ...the necessary Integrase protein in mammalian cells. The second device is a piece of linear DNA which is recognized by the Integrase protein and integrated i ...ign of the linear DNA is shown in Figure 2. The fabrication of this linear piece is detailed in the section explaining linear DNA amplification.21 KB (3,216 words) - 06:47, 12 December 2016
- ...enome DNA of this engineered phage, shown in Fig. 1, needs two functions. One is inducible expression of <i>g2p</i>. We thus designed our part to replac ...ith two modifications related to our design kept plaque forming activity. One is replacement of the promoter for <i>g2p</i> with a constitutive promoter,4 KB (640 words) - 16:30, 28 October 2013
- ...in pET28x. The GFP-CBD fusion protein is expressed and mixed with a gauze piece. The green fluorescent on the gauze is not significantly reduced after wash ...iluted to a certain concentration and mixed with gauze piece and the gauze piece is washed three times by ddH2O afterwards. As a result, the CBD protein bin7 KB (1,100 words) - 17:34, 1 November 2017
- ...in pET28x. The GFP-CBD fusion protein is expressed and mixed with a gauze piece. The green fluorescent on the gauze is not significantly reduced after wash ...luted to a certain concentration and mixed with gauze piece, and the gauze piece is washed three times by ddH2O afterwards. As a result, the CBD protein bin7 KB (1,158 words) - 17:40, 1 November 2017
- ...in pET28x. The GFP-CBD fusion protein is expressed and mixed with a gauze piece. The green fluorescent on the gauze is not significantly reduced after wash ...iluted to a certain concentration and mixed with gauze piece and the gauze piece is washed three times by ddH2O afterwards. As a result, the CBD protein bin7 KB (1,100 words) - 17:51, 1 November 2017
- ...in pET28x. The GFP-CBD fusion protein is expressed and mixed with a gauze piece. The green fluorescent on the gauze is not significantly reduced after wash ...iluted to a certain concentration and mixed with gauze piece and the gauze piece is washed three times by ddH2O afterwards. As a result, the CBD protein bin7 KB (1,070 words) - 17:50, 1 November 2017
- ...sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are ...tem is applied, namely gRNA, Cas9 effector protein, and repair template on one plasmid.13 KB (1,833 words) - 07:50, 20 October 2021
- ...sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are ...tem is applied, namely gRNA, Cas9 effector protein, and repair template on one plasmid.12 KB (1,804 words) - 14:02, 19 October 2021
- ...a coding sequence of left homology arm, refers to the flanking sequence on one side of the HXK1 sequence, which is completely consistent with the genome s ...coding sequence of right homology arm, refers to the flanking sequence on one side of the HXK1 sequence, which is completely consistent with the genome s12 KB (1,705 words) - 04:05, 22 October 2021
- This is a tRNA Mimicking Structure (TMS). It is a novel piece of synthetically designed RNA, which folds in the same manner as tRNA, init ...ll as a culture only induced by 1 mM IPTG, with no L-arabinose (IPTG), and one only induced by 0.1 % w/v L-arabinose, with no IPTG (L-ara).10 KB (1,639 words) - 14:34, 12 October 2023
- ...ing. I also export a pic with transparent background in .png format (right one). ...just the right way to get the perfect result - kind of like a puzzle. Each piece is different but they are all part of the whole system. The variety of colo12 KB (1,973 words) - 21:13, 27 May 2013
- ...since the band of Sortase A5M is a little higher than the one of Sortase A7M. ...ission maximum of TAMRA over time is also visible in the negative control. One61 KB (7,234 words) - 22:48, 21 October 2019
- ...ein at 100x under fluorescent microscope. A slight unipolar focus forms in one <i>E. coli</i> cell.]] ...gments were then spliced together by overlap extension yielding one linear piece of DNA which contained the entire construct.<br /><br />14 KB (2,223 words) - 23:45, 30 October 2017
- ...strain of the same batch. “U” is the amount of activity which releases one micromole of pNP per minute under these assay conditions. The error bar is ...n efficiency (11.39%), lower than the empty control’s in the same batch. One possible reason might be the positively charged L2NC would interact with ne13 KB (1,975 words) - 16:11, 13 October 2022
- ...e of TEVp and X-gal. The large red piece is the Omega-fragment, the purple piece is the Alpha-fragment, the yellow circle is TEVp. TEVp would cleave iGal an ...one transformation of <i>S. cerevisiae</i> with TEVp and each row makes up one transformation with the iGal. As negative control, no TEVp and iGal were ad8 KB (1,297 words) - 11:40, 12 October 2022