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  • Part:BBa K1172501
    ...ironment (Freulet-Marrière et al., 2000). OprF consists of two domains: a C-terminal periplasmic domain and a N-terminal domain that forms an eight-str ...ctive at a temperature range of 20°C and 45°C (Lee et al., 2005). At 37°C, whole cell lipase activity remains over 80% in cells incubated in Tris-HCl
    56 KB (7,913 words) - 12:22, 10 October 2023
  • Part:BBa K1980001
    ...n to the periplasm and added a C terminal sfGFP fluorescent protein with a C terminal his tag. The Csp1 and sfGFP are separated by a short hydrophilic, ...in we purified lacked the chelator portion. We attached the his tag to the C terminus of sfGFP rather than the N terminus of Csp1 in order to preserve t
    9 KB (1,507 words) - 21:17, 24 October 2016
  • Part:BBa K1980010
    ...tains the bacterial copper chelator Csp1 (Copper Storage Protein 1) with a C-terminal sfGFP tag (connected with short hydrophilic flexible linker) behin ...n for 3-4hrs (until entrance into the exponential growth phase) at 37°C and 225rpm shaking in the presence of the inducer before measurement. This
    12 KB (1,897 words) - 20:59, 24 October 2016
  • Part:BBa K1980000
    ...om the E. coli enzyme CueO in place of the native TAT sequence and added a C terminal his tag to aid purification.</p> <p>We purified this a version of this part with a C-terminal His tagged-sfGFP (<a href="https://parts.igem.org/Part:BBa_K198000
    6 KB (982 words) - 21:18, 24 October 2016
  • Part:BBa K4147004
    ...s expected. C) Amplification of DrsB1 Construct with LacI Promoter at 55 °C as temperature of alignment where the highlighted band corresponds to appro [2] Nicolas, P., & el Amri, C. (2009). The dermaseptin superfamily: A gene-based combinatorial library of
    4 KB (560 words) - 06:13, 10 October 2022
  • Part:BBa K1980000:Design
    ...olved in copper homeostasis. There is a hexahistidine purification tag on C-terminus in order to purify the protein whilst preserving the N-terminal TA Nicolas Vita, Semeli Platsaki, Arnaud Basle, Stephen J. Allen, Neil G. Paterson, An
    1 KB (191 words) - 20:03, 23 October 2016
  • Part:BBa K1980001:Design
    ...and sfGFP to fold separately. There is a hexahistidine purification tag on C-terminus in order to purify the protein whilst preserving the N-terminal TA Nicolas Vita, Semeli Platsaki, Arnaud Basle, Stephen J. Allen, Neil G. Paterson, An
    1 KB (215 words) - 20:02, 23 October 2016
  • Part:BBa K1980010:Design
    ...olved in copper homeostasis. There is a hexahistidine purification tag on C-terminus of Csp1 in order to purify the protein whilst preserving the N-ter Nicolas Vita, Semeli Platsaki, Arnaud Basle, Stephen J. Allen, Neil G. Paterson, An
    2 KB (290 words) - 21:04, 24 October 2016
  • Part:BBa K2486108
    [2] Nicolas, P., & el Amri, C. (2009). The dermaseptin superfamily: A gene-based combinatorial library of
    4 KB (502 words) - 01:51, 22 October 2021
  • Part:BBa K098997
    ...at loses binding efficiency with the cI promoter between 35 and 42 degrees C. We introduce the mRFP to check if the system works at 37ºC and not under 20ºC (used as a reporter).
    6 KB (834 words) - 19:21, 1 November 2017
  • Part:BBa K2577001
    [2] Nicolas, P., & el Amri, C. (2009). The dermaseptin superfamily: A gene-based combinatorial library of
    4 KB (498 words) - 01:50, 22 October 2021
  • Part:BBa K4147009
    [2] Nicolas, P., & el Amri, C. (2009). The dermaseptin superfamily: A gene-based combinatorial library of
    2 KB (231 words) - 06:09, 10 October 2022
  • Part:BBa K4768002
    <li><sup>[1]</sup>Chabrol E, Stojko J, Nicolas A, et al. VHH characterization.Recombinant VHHs: Production, characterizati <li><sup>[3]</sup>Chabrol E, Fagnen C, Landron S, et al. Biochemistry, structure, and cellular internalization of
    8 KB (1,013 words) - 08:26, 11 October 2023