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  • Part:BBa K5357016
    An alphafold 2 model of nanobody-linker-NanoLuciferase (NB-NL). ...ried out protein expression, but no protein was expressed (no bands in the NB-NL lanes).
    9 KB (1,428 words) - 20:52, 1 October 2024
  • Part:BBa K5357022
    ...t is hopefully a correctly folded nanobody- NanoLuciferase-NanoLuciferase (NB-NL-NL) conjugate, which can be purified using its 6xHis tag (see BBa_K53570 ...sequencing because the plasmids were assembled with the coding sequence of NB-NL-NL inserted by Genscript.
    8 KB (1,162 words) - 20:58, 1 October 2024
  • Part:BBa K4275026
    ...oyed the surface display system neae(intimin) to present three pairs of Ag/Nb on the ''E. coli'' 's cell membrane and assessed their strengths. Simultane Neae-Nb is a type of nanobody, which contains variable domains of camelid heavy-cha
    8 KB (1,203 words) - 11:10, 12 October 2023
  • Part:BBa K4275030
    ...odified in the construction of pRha-riboJ-Neae-Nb to enhance expression of Nb which enables cellulosome complex to be displayed on surface of <i>E.coli</ <h3>The Expression of pRha-riboJ-Neae-Nb</h3>
    5 KB (674 words) - 11:15, 13 October 2022
  • Part:BBa K4768002
    ...with a His-tag for purification. The signal peptide was fused to Anti-HER2-nb for periplasmic targeting, which promotes disulfide bond formation.</p> <p><I>E. coli</I> expression of Anti-HER2-nb was induced using IPTG, and purification was carried out using Cobalt resin
    8 KB (1,013 words) - 08:26, 11 October 2023
  • Part:BBa K914003
    ...odified in the construction of pRha-riboJ-Neae-Nb to enhance expression of Nb which enables cellulosome complex to be displayed on surface of <i>E.coli</ <b>The Expression of pRha-riboJ-Neae-Nb</b>
    25 KB (3,858 words) - 05:12, 30 September 2024
  • Part:BBa K4768001
    ...e PCR products of <i>tymp, sfgfp</i>, and <i>anti-HER2 nanobody (anti-HER2-nb)</i> as templates for expression with the PURE<I>frex</I> 2.0 kit (See the ...size of 52 kDa, <i>sfGFP</i> with an expected size of 26 kDa, <i>anti-HER2-nb</i> with an expected size of 17 kDa, protein ladder.</i></span></figcaption
    15 KB (2,152 words) - 16:51, 10 October 2023
  • Part:BBa K4765106
    ..._K4765105 BBa_K4765105] .In our project, we took full advantage of the Ag-Nb interaction to create a bacteria lawn with a programmable physical structur To confirm biofilm formation through intimin-Ag/Nb, we employed both '''aggregation experiments''' and '''fluorescence microsc
    7 KB (951 words) - 15:52, 12 October 2023
  • Part:BBa K4768000
    ...ells (sample 1). In a second protocol, liposomes pre-coated with anti-HER2-nb were injected in the growth medium on top of Caco-2 cells, where they have ..."titre-image"><i><b>Figures 9: Fluorescence microscopy images of anti-HER2-nb- and folate-decorated liposomes on top of Caco-2 cells (sample 1).</b> a) I
    20 KB (2,891 words) - 21:44, 10 October 2023
  • Part:BBa K5466020
    <H2><font color="#012d5c"><b>Display of Anti-AFB1 Nb</b></font></H2> Displaying the NB28-S102D nanobody (Nb) on the yeast cell wall via Aga2P allows the engineered yeast to maximize i
    8 KB (1,213 words) - 19:48, 1 December 2024
  • Part:BBa K1185000
    ...' chromosomes, some colonies were picked from Plate 1 and innoculated into NB/MSM media with xylose concentration varying between 0% and 0.8% and incubat ...rmed with our switch BioBrick after being innoculated into a 1:1 ration of NB and MSM media with a range of xylose concentrations and incubated in 30oC f
    15 KB (2,148 words) - 12:08, 26 October 2013
  • Part:BBa K2109102:Design
    ...ody (sdAb) selection system. It contains the previously characterized λcl-Nb fusion protein and the coding sequence of the anti-GFP sdAb (BH-GBP3). This ...terized () therefore we anticipate that it will bind only to GFP. The λcl-Nb (BH-GBP3) fusion protein will bind to the λcl DNA binding domain located u
    2 KB (302 words) - 02:32, 28 October 2016
  • Part:BBa K4439001
    nb(circles) =[360/(2*alpha)] (2) nb(circles for width) = [W2 * r(proteins)] (4)
    7 KB (1,007 words) - 13:40, 12 October 2022
  • Part:BBa K4439007
    nb(circles) =[360/(2*alpha)] (2) nb(circles for width) = [W2 * r(proteins)] (4)
    22 KB (3,475 words) - 15:17, 12 October 2022
  • Part:BBa K4439013
    nb(circles) =[360/(2*alpha)] (2) nb(circles for width) = [W2 * r(proteins)] (4)
    18 KB (2,796 words) - 13:43, 12 October 2022
  • Part:BBa K4439015
    nb(circles) =[360/(2*alpha)] (2) nb(circles for width) = [W2 * r(proteins)] (4)
    16 KB (2,582 words) - 15:20, 12 October 2022
  • Part:BBa K5466017
    <H2><font color="#012d5c"><b>Display of Anti-AFB1 Nb</b></font></H2> Displaying the NB28-S102D nanobody (Nb) on the yeast cell wall via Aga2P allows the engineered yeast to maximize i
    6 KB (885 words) - 21:18, 1 October 2024
  • Part:BBa J428074
    ...<img src='https://static.igem.wiki/teams/5088/registry/contribution/ruby-nb.png'> <img src='https://static.igem.wiki/teams/5088/registry/contribution/nb-ruby-spectrum.png' width=600px>
    18 KB (2,654 words) - 22:10, 1 October 2024
  • Part:BBa K5466035
    <H2><font color="#012d5c"><b>Display of Anti-AFB1 Nb</b></font></H2> Displaying the NB28-S102D nanobody (Nb) on the yeast cell wall via Aga2P allows the engineered yeast to maximize i
    6 KB (875 words) - 19:58, 1 December 2024
  • Part:BBa K3930026
    ...e production of 3,6-nonadienal and LacI repressor. The sequences of the <i>Nb-9-LOX</i> and the <i>Cm-9-HPL</i> were codon optimized for expression into ...ss="pr-0" target="_blank">(BBa_K3930029)</a>, driving the expression of <i>Nb-9-LOX</i> and <i>Cm-9-HPL</i>.</p>
    6 KB (863 words) - 06:47, 17 October 2021

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