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  • Part:BBa K4696509
    ...pression of the Chimeric Antigen Receptor (CAR) and transfected macrophage M1 polarization in a scalable and controlled manner. ...bining L7Ae with DDd, a degradation domain evolved from an enzyme found in E. coli, the L7Ae-DDd complexes are specifically designed to degrade rapidly
    11 KB (1,540 words) - 15:21, 12 October 2023
  • Part:BBa K4696507
    ...pression of the Chimeric Antigen Receptor (CAR) and transfected macrophage M1 polarization in a scalable and controlled manner. ...bining L7Ae with DDd, a degradation domain evolved from an enzyme found in E. coli, the L7Ae-DDd complexes are specifically designed to degrade rapidly
    11 KB (1,582 words) - 15:21, 12 October 2023
  • Part:BBa K4696508
    ...pression of the Chimeric Antigen Receptor (CAR) and transfected macrophage M1 polarization in a scalable and controlled manner. ...bining L7Ae with DDd, a degradation domain evolved from an enzyme found in E. coli, the L7Ae-DDd complexes are specifically designed to degrade rapidly
    11 KB (1,585 words) - 15:21, 12 October 2023
  • Part:BBa K2871000
    ...id long signal peptide derived from the map gene from the Enteropathogenic E. coli strain E22. ...ibed by Charpentier & Oswald (2004) to be sufficient to target proteins to E. coli type III secretion pathway. A small 4xGS-linker is added for added av
    14 KB (2,087 words) - 15:07, 26 October 2020
  • Part:BBa K4696505
    ...e for CAR-M’s functionality. We designed two version of CAR construct, i.e. CAR per se and CAR – P2A – IFN-γ. The yellow part shown in the above ...art-registry/part-registry/part-registry/part-registry/part-registry/m0-to-m1.png" width="350"</html>
    6 KB (872 words) - 14:01, 12 October 2023
  • Part:BBa K4696506
    ...e for CAR-M’s functionality. We designed two version of CAR construct, i.e. CAR per se and CAR – P2A – IFN-γ. The yellow part shown in the above ...art-registry/part-registry/part-registry/part-registry/part-registry/m0-to-m1.png" width="350"</html>
    6 KB (871 words) - 14:00, 12 October 2023
  • Part:BBa K3440010
    ...molecule used in our system to communicate between our detection module in E. coli and our electrical output module in Shewanella oneidensis. After insertion into a pSB1C3 plasmid and transformation of E. coli TOP10 cells using heat shock, we picked colonies corresponding to thi
    3 KB (476 words) - 23:22, 27 October 2020
  • Part:BBa K1140006:Experience
    The team observed that this part works correctly in ''E. coli'' K12. The synthetic RNAT proved to regulate expression in response t .... Temperature dependence of mCherry translation by u6 RNA thermometer in ''E. coli''.''' Tet repressor is NOT present in this test. Two control cultures
    4 KB (595 words) - 21:17, 28 October 2013
  • Part:BBa K5062008
    ...i-tumor M1 state while guiding tumor-associated macropages from M2 towards M1 as well. *Human IFNγ Armouring Domain allowing for the sustained M1 poloarization of macrophages enforcing their anti-tumor effect (Jorgovanovi
    5 KB (738 words) - 11:37, 27 September 2024
  • Part:BBa K5062007
    ...i-tumor M1 state while guiding tumor-associated macropages from M2 towards M1 as well. *Human IFNγ Armouring Domain allowing for the sustained M1 poloarization of macrophages enforcing their anti-tumor effect (Jorgovanovi
    7 KB (916 words) - 05:34, 28 September 2024
  • Part:BBa K4003005
    ...ure shows that PPM1A can reduce the expression of specific markers of type M1 inflammatory macrophages and inhibit the production of inflammatory factors ...ure shows that PPM1A can reduce the expression of specific markers of type M1 inflammatory macrophages and inhibit the production of inflammatory factors
    6 KB (917 words) - 09:04, 19 October 2021
  • Part:BBa K5160111
    ...of the experiment, we explored its expression in the prokaryotic organism E. coli. ...des a detailed description of the characterization of brazzein produced in E. coli this year. We hope that these characterizations can provide the iGEM
    12 KB (1,864 words) - 05:07, 1 October 2024
  • Part:BBa K4040003
    ...flammatory cytokines and chemokines, converted bystander M2 macrophages to M1, upregulated antigen presentation machinery, recruited and presented antige ...cates p<0.01, *** indicates p<0.0001 by two-tailed Fisher Exact Test(a and e) or by Ordinary one way ANOVA with Dunnet’s correction for multiple compa
    16 KB (2,413 words) - 10:41, 13 October 2021
  • Part:BBa K5160112
    ...genome to enable heterologous expression of the sweet protein Thaumatin in E. coli BL21(DE3).<br> ...of Thaumatin, we can induce the expression of the target gene Thaumatin in E. coli. For detailed information on this component, please refer to <html><a
    12 KB (1,746 words) - 05:16, 1 October 2024
  • Part:BBa K4390077
    ...ater bottle at 50 ºC. For highly crystalline PET, a simple pre-treatment (e.g., melting) allows the PET to be feasibly degraded. ...SSGSGSESKST). L2NC is a truncated version of the L2 ribosomal protein from E. coli, designed for fusion to C-terminal of a protein using JUMP assembly.
    13 KB (1,975 words) - 16:11, 13 October 2022
  • Part:BBa K3997000
    ..., and it is crucial for hydrolysis of PET. To verify this property, we use E. coli as the starting strain and construct an engineered strain of IsPETase ...the function of the part, the IsPETase and MHETase gene were expressed in E. coli under the control of T7 promoter. Then the bacterial cells are collec
    15 KB (2,268 words) - 13:30, 12 October 2022
  • Part:BBa K5160003
    ...coli</em> by constructing expression vectors and transformed them into <em>E. coli</em>, then induced the expression with IPTG and tested the protein le ...in our expression vector. Subsequently, we transformed the vector into <em>E. coli</em> BL21(DE3) and picked the colonies for amplification on the mediu
    46 KB (6,947 words) - 13:55, 2 October 2024
  • Part:BBa K2013003
    ...zation before synthesizing it to encode the target product successfully in E. coli. After the assembly ,the plasmid was transferred into the Competent E. coli top10. After culturing overnight in LB,we minipreped the plasmid for
    13 KB (1,905 words) - 02:29, 22 October 2021
  • Part:BBa K4003000
    ...ure shows that PPM1A can reduce the expression of specific markers of type M1 inflammatory macrophages and inhibit the production of inflammatory factors ...ity assay with pNPP as the substrate. All data were presented as mean ± S.E.M (*P<0.05, **P< 0.01, ***P< 0.001).
    7 KB (970 words) - 08:36, 19 October 2021
  • Part:BBa K4865002
    ...is a secretion signal, pelB, which directs synthesized polypeptides to the E. coli periplasm (Yoon S.H. et al., 2010). ...cloning technique. The recombinant expression vector was transformed into E. coli BL21 competent recipient cells and used for subsequent protein expres
    16 KB (2,392 words) - 04:45, 24 September 2024

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