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  • ...e more soluble than TEV and the other proteases. Also, if it is shown that e.g dCas9-nTEV or dCas9-cTEV would misfold, or there exist other complication [2] Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca
    5 KB (806 words) - 11:44, 12 October 2022
  • [4] Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca ...ang, Yueli Yun, Shihui Yang, Guimin Zhang & Li Yi (2021). A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia
    6 KB (977 words) - 12:39, 12 October 2022
  • [4] Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca ...ang, Yueli Yun, Shihui Yang, Guimin Zhang & Li Yi (2021). A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia
    6 KB (976 words) - 12:52, 12 October 2022
  • ...for downstream SDS-PAGE analysis. As a control, EGFP, which is soluble in E.coli, is also expressed and analyzed with the same protocol. 1. Fink, T., Lonzarić, J., Praznik, A., Plaper, T., Merljak, E., Leben, K., Jerala, N., Lebar, T., Strmšek, Ž., Lapenta, F., Benčina, M
    6 KB (888 words) - 13:26, 2 October 2024
  • ...for downstream SDS-PAGE analysis. As a control, EGFP, which is soluble in E.coli, is also expressed and analyzed with the same protocol. 1. Fink, T., Lonzarić, J., Praznik, A., Plaper, T., Merljak, E., Leben, K., Jerala, N., Lebar, T., Strmšek, Ž., Lapenta, F., Benčina, M
    7 KB (983 words) - 11:37, 28 September 2024
  • ...for downstream SDS-PAGE analysis. As a control, EGFP, which is soluble in E.coli, is also expressed and analyzed with the same protocol. ..., i.e., proteins in the supernatant after ultrasonication; Lane3, FS1-1, i.e., proteins in the final supernatant. MW of TEV is around 31.0 KDa. This res
    5 KB (805 words) - 11:12, 28 September 2024
  • ...for downstream SDS-PAGE analysis. As a control, EGFP, which is soluble in E.coli, is also expressed and analyzed with the same protocol. 1. Fink, T., Lonzarić, J., Praznik, A., Plaper, T., Merljak, E., Leben, K., Jerala, N., Lebar, T., Strmšek, Ž., Lapenta, F., Benčina, M
    7 KB (983 words) - 13:28, 2 October 2024