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• Part:BBa K2595004
  ...of spf expression. (GA Hansen et al., 2012) Studies also reveal that Spot 42 is likely to interact with mK2 mRNA and affect galT expression, which could ...ion with this restriction enzyme and ligation with a target sequence, Spot 42 can regulate the expression of the gene that contains the complementary reg
  2 KB (263 words) - 14:27, 17 October 2018
• Part:BBa K4165005
  Amyloid β Protein Fragment 1-42 which results from the amyloidogenic degradation of Amyloid beta precursor ...the cleavage of the remaining part of APP to form A𝛽-42 fragments. The 42 amino acid fragments are toxic in nature and form A𝛽 plaques when aggreg
  4 KB (513 words) - 13:52, 13 October 2022
• Part:BBa K2671000
  ...e GS-linker sequence where a SpeI-site was added and the sequence for Aβ1-42 was cleaved off. With these improvements mNG can now be used as a reporter ...sion tag. From Figure 1. you can clearly see that the version without Aβ1-42 folds better and thus gives a much higher fluorescence, while the fusion ve
  5 KB (760 words) - 09:52, 20 October 2019
• Part:BBa K608351:Experience
  ...352 BBa_K608352]), and also at different temperatures (probably a 25°C to 42°C gradient). ...on the right show the same plates under blue light, where only the 37 and 42 Celsius glowed. This indicates that GFP was only found at or above 37 Celsi
  6 KB (974 words) - 13:15, 27 October 2016
• Part:BBa K1053006:Experience
  <BR>pSB1A3-P_BAD-TR(42)HHR-taR12-DT<BR> <BR>pSB1A3-Pconst(H)-taR42-DT-P_BAD-TR(42)HHR-taR12-DT<BR>
  3 KB (479 words) - 01:36, 28 September 2013
• Part:BBa K1053009:Experience
  <BR>pSB1A3-P_BAD-TR(42)HHR-taR12-DT<BR> <BR>pSB1A3-Pconst(H)-taR42-DT-P_BAD-TR(42)HHR-taR12-DT<BR>
  3 KB (465 words) - 04:16, 28 September 2013
• Part:BBa K2446040
  ...an synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 42-10 (ZF_42-10) as protein chassis. [[#References|[1]]]. ZF_42-10_KRAB (or TF [[file:T--Fudan--42-10.png|500px|middle|'''Figure 2''': the testing circuits of ZF_42-10-KRAB&
  8 KB (1,240 words) - 12:03, 1 November 2017
• Part:BBa K3017003
  ...osed of 2 functional domains, extensor, for linearizing the loop, and Spot 42, aiding the stability of the asRNA.</p> <h2>Spot 42</h2>
  10 KB (1,652 words) - 01:36, 22 October 2019
• Part:BBa K3017004
  ...osed of 2 functional domains, extensor, for linearizing the loop, and Spot 42, aiding the stability of the asRNA.</p> <h2>Spot 42</h2>
  10 KB (1,652 words) - 01:35, 22 October 2019
• Part:BBa K3017064
  ...osed of 2 functional domains, extensor, for linearizing the loop, and Spot 42, aiding the stability of the asRNA.</p> <h2>Spot 42</h2>
  11 KB (1,723 words) - 01:42, 22 October 2019
• Part:BBa K3017060
  ...osed of 2 functional domains, extensor, for linearizing the loop, and Spot 42, aiding the stability of the asRNA.</p> <h2>Spot 42</h2>
  11 KB (1,724 words) - 01:41, 22 October 2019
• Part:BBa K4252023
  ...ransferred into cI857,phlF,R promoter and mCherry at 30℃,33℃,37℃ and 42℃,in order to verify that the temperature sensitive regulating element is ...he resistant culture dish and cultivate them at 30 ℃, 33 ℃, 37 ℃ and 42 ℃ for 12h and 15h.
  10 KB (1,474 words) - 15:12, 12 October 2022
• Part:BBa K4634018
  Here we hope to optimize PL promoter, enabling a higher expression level at 42 °C. The following content mainly describes the pBV220_PL2 which shows prom ...ering the occurrence of transcription. When the temperature rises to about 42 °C, the repressor is thus denatured and the transcription happens.[1]
  14 KB (1,796 words) - 14:06, 12 October 2023
• Part:BBa K993002:Experience
  ...ure in which the IbpB RNA Thermometer would properly thermosense which was 42 C; we chose the second temperature as one that there would be no RFP transl ...separated for incubation in the 2 different temperatures. The cultures in 42 C were incubated for 24 hours and the cultures in 27 C were incubated for 3
  5 KB (813 words) - 07:41, 29 June 2013
• Part:BBa K1033906
  <td>42 44 92 150 175 179 181 198 219</td> <td>42 44 92 150 175 179 181 198 219</td>
  16 KB (2,179 words) - 01:51, 22 October 2019
• Part:BBa K2009430
  ...intensity of two groups of S. cerevisiae cultivated separately at 30,34,38,42 ℃ without GdnHCl. The first group was cultivated for 1 hour and the other ...of the aggregation of Sup35. According to Zhang et al, from 30 degrees to 42 degrees, the fluorescence intensity decreases for about 20%, owing to the e
  11 KB (1,660 words) - 09:08, 19 October 2016
• Part:BBa K2009430:Experience
  ...intensity of two groups of S. cerevisiae cultivated separately at 30,34,38,42 ℃ without GdnHCl. The first group was cultivated for 1 hour and the other ...of the aggregation of Sup35. According to Zhang et al, from 30 degrees to 42 degrees, the fluorescence intensity decreases for about 20%, owing to the e
  8 KB (1,338 words) - 09:01, 19 October 2016
• Part:BBa K2009701
  ...intensity of two groups of S. cerevisiae cultivated separately at 30,34,38,42 ℃ without GdnHCl. The first group was cultivated for 1 hour and the other ...of the aggregation of Sup35. According to Zhang et al, from 30 degrees to 42 degrees, the fluorescence intensity decreases for about 20%, owing to the e
  9 KB (1,250 words) - 09:03, 19 October 2016
• Part:BBa K2009701:Experience
  ...intensity of two groups of S. cerevisiae cultivated separately at 30,34,38,42 ℃ without GdnHCl. The first group was cultivated for 1 hour and the other ...of the aggregation of Sup35. According to Zhang et al, from 30 degrees to 42 degrees, the fluorescence intensity decreases for about 20%, owing to the e
  6 KB (925 words) - 09:03, 19 October 2016
• Part:BBa K2505004
  ...cription''' <br style="clear: both" />After mixing, samples were heated at 42° C. for 20 min, 99 ° C. for 5 min, 4 ° C. for 5 min, then stored at -20 ...thylallyl diphosphate:ATP/ADP isopentenyltransferases. Plant Cell Physiol. 42:677-85.
  8 KB (1,230 words) - 18:24, 1 November 2017