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Combined display of all available logs of parts.igem.org. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

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  • 21:51, 31 October 2017 Ignasmazelis (Talk | contribs) uploaded File:5plasmidresult.jpg (SynORI 5 plasmid co-transformation results 1 - No trigger 1 (control). 2 - No trigger 2 (control). 3 - No lambda activator plasmid (control). 4 - Full System: lambda activator plasmid; toehold 1 alpha-neo; toehold 2 beta-neo; trigger 1; trigger 2.)
  • 21:29, 31 October 2017 Ignasmazelis (Talk | contribs) uploaded File:5plasmid.png (The schematic representation of 5 plasmid SynORI selection system. First plasmid constantly expresses lambda, the activator of the modified phage promoter, which controls the expression of the Trigger 1 and 2. The Triggers unlock the translation of spl...)
  • 21:14, 31 October 2017 Ignasmazelis (Talk | contribs) uploaded File:4plasmid.png (The schematic representation of 4 plasmid SynORI selection system. First 2 plasmids constantly express Toehold 1 and 2 which are coupled with the split antibiotic alpha and beta subunits but has its translation locked. Trigger 1 and 2 are constantly ex...)
  • 17:13, 31 October 2017 Ignasmazelis (Talk | contribs) uploaded File:Alfabeta.jpg (2 vectors with constant expression of alpha and beta subunits were co-transformed into TG1 competent cells and plated on agar with kanamycin antibiotic)
  • 12:09, 31 October 2017 Ignasmazelis (Talk | contribs) uploaded File:Toehold.png (Toehold switches repress translation through base pairs programmed before and after the start codon (AUG), leaving the RBS and start codon regions completely unpaired. The toehold domain a binds to a complementary a* domain on the trigger RNA ant initi...)