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Combined display of all available logs of parts.igem.org. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 19:12, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:AlphaFold dcas9-splitHRV3C.png (A simulation in AlphaFold where nHRV_3C (red) and cHRV_3C (cyan) are bound to dCas9.)
- 19:03, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Alternative dCas9-splitProteases.png (The following proteases are bound to dCas9 and are displayed in different colors. nPPVp is yellow, nSbMV is red, nSuMMV is blue.)
- 17:36, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:System dCas9-splitTEV and iGal.png (Illustration of how the full system would look like. How dCas9-splitTEV acts as a DNA sensor and activates the reporter protein iGal to produce a blue pigment as a visible readout.)
- 17:33, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:System dCas9-splitTEV and iGal.png (Illustration of how the full system would look like. How dCas9-splitTEV acts as a DNA sensor and activates the reporter protein iGal to produce a blue pigment as a visible readout.)
- 17:20, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Schematic dCas9-splitTEV.png (A schematic of dCas9-splitTEV with gRNA and binding to DNA, inducing complementation of the split TEV.)
- 13:58, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:TEVp PPVp SbMVp SuMMVp HRV3C.png (List of proteases (TEVp, PPVp, SbMVp, SuMMVp, HRV3C) and their recognition sites.)
- 11:32, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Experiment iGal TEV Xgal PBS.png (An experiment that combined cell-lysates of S. cerevisiae transformed with iGal and TEVp in a buffer of PBS containing X-gal. Going grom left to right, the pictures show how the experiment looks after 1, 2, and 5 days since its start.)
- 11:18, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Experiment iGal TEV Xgal MQ.png (An experiment that combined cell-lysates of S. cerevisiae transformed with iGal and TEVp in a buffer of MilliQ containing X-gal. Going grom left to right, the pictures show how the experiment looks after 1, 2, 3, and 6 days since its start.)
- 10:48, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:Comparison iGal vs B-gal.png (The red sequence in iGal is the Omega-fragment and the pink and purple sequence is the Alpha-fragment. The GS-linker is white and the recognition site for TEVp is yellow. The pink sequence in B-gal is the part that is missing ín the Omega-fragment, an...)
- 10:46, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Comparison iGal vs B-gal.png (iGal (left) compared to B-gal. The red sequence in iGal is the Omega-fragment and the pink and purple sequence is the Alpha-fragment. The GS-linker is white and the recognition site for TEVp is yellow. The pink sequence in B-gal is the part that is m...)
- 10:42, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:System iGal.png (A schematic on how the iGal system is supposed to function when TEVp and X-gal is present.)
- 10:37, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:System iGal.png (A schematic on how the iGal system is supposed to function when TEVp and X-gal is present.)
- 10:31, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:System iGal.png (A schematic on how the iGal system is supposed to function when TEVp and X-gal is present.)
- 10:30, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:System iGal.png (A schematic on how the iGal system is supposed to function when TEVp and X-gal is present.)
- 10:24, 11 October 2022 AndersKallberg (Talk | contribs) uploaded a new version of File:Composition iGal.png (The figure displays how the Alpha- and Omega-fragments from Beta-galactosidase are rearanged and fused with a linker that can be cleaved by TEVp.)
- 10:22, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Composition iGal.png (The figure displays the Alpha- and Omega-fragments from Beta-galactosidase are rearanged and fused with a linker that can be cleaved by TEVp.)
- 10:11, 11 October 2022 AndersKallberg (Talk | contribs) uploaded File:Background iGal.png (Visual comparison of the components of Inhibited Beta-galactosidase to Beta-galactosidase)